Electrochemical biosensor based on strand displacement reaction for on-site detection of Skeletonema costatum

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-13 DOI:10.1007/s00604-025-06966-9

Yaling Liu, Yibo Zhang, Changrui Ye, Yayi He, Shaopeng Wang, Man Zhang, Hao Fu, Shan Lu, Liwei Wang

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Abstract

An electrochemical biosensor was constructed for quantitatively detecting Skeletonema costatum (S. costatum) by combining the electrode modification material (NC-Au) with a strand displacement reaction (SDR). The SDR process addresses the issues of steric hindrance and electrostatic repulsion resulting from the large size of genomic DNA. It enhances the efficiency of the interfacial hybridization reaction and endows the biosensor with remarkable sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) were 33.43 fg/μL (831 cells/L) and 87.21 fg/μL (2112 cells/L), respectively. Additionally, the biosensor has demonstrated excellent accuracy compared to methods such as microscopy and ddPCR (P > 0.05). Subsequent assessment of the adjacent waters of the Beibu Gulf using biosensors indicated a low risk of S. costatum red tide outbreaks in the region, which is consistent with the findings of the ecological survey. Therefore, we believe that this biosensor can provide a completely new idea for the dynamic monitoring and early warning of S. costatum red tide.

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基于链位移反应的电化学生物传感器现场检测骨骸

将电极修饰材料（NC-Au）与链位移反应（SDR）相结合，构建了用于定量检测骨骸藻（S. costatum）的电化学生物传感器。SDR过程解决了由大尺寸基因组DNA引起的空间位阻和静电排斥问题。它提高了界面杂交反应的效率，使生物传感器具有显著的灵敏度。检测限和定量限分别为33.43 fg/μL（831个细胞/L）和87.21 fg/μL（2112个细胞/L）。此外，与显微镜和ddPCR等方法相比，生物传感器具有出色的准确性（P > 0.05）。随后，利用生物传感器对北部湾附近海域进行了评估，结果表明该区域发生海螺赤潮的风险较低，这与生态调查结果一致。因此，我们相信这种生物传感器可以为海参赤潮的动态监测和预警提供一种全新的思路。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.