Origins and Diversification of Myiasis Across Blowflies

Abstract

Parasitism represents a prevalent and successful ecological strategy that has evolved independently numerous times across metazoa. Understanding the origin and diversification of parasitism is a central question in evolutionary biology. This study investigated the evolutionary path leading to a specific form of parasitism in blowflies known as myiasis, where larvae develop on or within a vertebrate. We modeled myiasis-associated traits, including trophic specialization (obligatory parasitism, facultative parasitism and saprophagy), larval food substrate (necrotic, fresh or both) and developmental temperature (constant, variable or both) across the blowfly phylogeny. Our results suggested that the ancestral state of blowflies likely encompassed saprophagy or facultative parasitism, with larvae developing in corpses or necrotic tissues from wounds in either homeothermic or heterothermic hosts. Furthermore, our analysis highlights the role of facultative parasitism as an intermediate step for obligate parasitism in blowflies, indicating that pre-adaptations for a facultative parasitic lifestyle may serve as stepping stones for emerging obligate parasitism. These findings shed light on the complex evolutionary history of blowfly vertebrate parasitism, emphasizing the importance of facultative parasitism as a critical transitional stage in this evolutionary process.