OsLC1, a transaldolase, regulates cell patterning and leaf morphology through modulation of secondary metabolism

Abstract

Leaf morphogenesis is a crucial process in plants that governs essential physiological functions such as photosynthesis and transpiration. Despite significant advances in understanding leaf development, the mechanism of intricate cellular patterning remains elusive. We characterize the OsLC1 mutant, which displays a curly leaf phenotype alongside reductions in plant height and tiller number, which are indicative of multiple morphological abnormalities. Through map-based cloning, we identified OsLC1 as encoding a transaldolase (TA) protein, whose genetic variations in OsLC1 lead to the disruptions of cell patterning across the vasculature, bundle sheath cells, mesophyll, stomata, bulliform cells and sclerenchyma cells. OsLC1 exhibited TA activity and modulated metabolic flux to the shikimic pathway, thereby affecting phenylpropanoid metabolism. This regulation influenced lignin and flavonoid biosynthesis, ultimately modulating cellular pattern formation through perturbations to flavonoid-mediated auxin or lignin homeostasis. Notably, loss of OsLC1 function led to a reduction in leaf water status, which, along with abnormal cellular patterns in oslc1, caused leaf curling. Overall, our findings provide insights into the regulatory mechanisms underlying cell patterning in the leaf and offer valuable perspectives on leaf morphogenesis in rice.