Multiplex analysis of ovarian cancer patients using glycan microarray

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2025-02-11 DOI:10.1016/j.ab.2025.115806

Vadim S. Pokrovsky , Louay Abo Qoura , Aleksei A. Tikhonov , Alla Yu Rubina , Nikolai E. Kushlinskii

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引用次数: 0

Abstract

Investigation of tumor-associated glycan antigens (TAGs) could be helpful for the development of sensitive cancer diagnostics and novel therapies. Glycan microarrays are effective methods for analyzing glycans and anti-glycan antibodies, which are immobilized arrays of oligo- or poly-saccharides on different substrates, making them a promising class of oncological biomarkers. Blood serum samples from patients (n = 203) with ovarian cancer (OvaCan) and healthy volunteers were analyzed using a glycan microarray containing 63 immobilized glycans to determine changes in anti-glycan IgG and IgM antibody profiles in OvaCan. Levels of anti-glycan IgG and IgM antibodies in OvaCan statistically differed from levels in healthy donors: the most prominent statistically significant difference for anti-glycan IgG antibodies was found for 6-O-su-Le^c (AUC = 0.657, Se = 48.0 %, and Sp = 73.3 %). The AUC values for certain glycans investigated in diagnosing OvaCan indicated a fingerprint consisting of IgM antibodies to specific glycans, and the most specific anti-glycan IgM antibodies were Le^y (AUC = 0.625, Se = 98.0 % and Sp = 45.0 %). The potential of these serological biomarkers to distinguish between OvaCan and other malignancies is still an unresolved issue that requires more large-scale studies to confirm and validate the use of these biomarkers in the diagnosis of different types of cancer.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.