Temperature-responsive properties of pH-sensitive poly(methacrylic acid)-grafted brush coatings with controlled wettability for cell culture†

Abstract

Poly(methacrylic acid) (PMAA) is a well-known pH-responsive polymer with under-explored temperature-responsive properties. This study investigated the temperature-responsive properties of PMAA-grafted brush coatings, synthesized via the SI-ATRP polymerization of sodium methacrylate (NaMAA) and methacrylic acid (MAA) on glass surfaces. Distinct water contact angles were observed for PMAA brush coatings fabricated from NaMAA (38 deg) and MAA (60 deg) solutions. The reduced wettability of PMAA brushes from MAA indicates a reduced exposure of the hydrophilic moieties acquired during synthesis, which is postulated to occur with a lower grafting density. PMAA brush coatings showed a lower critical solution temperature (LCST), characterized by changes in wettability and thickness; however, this transition was not observed after immersion in various pH buffer solutions. Although inhibited growth of cells cultured on PMAA brushes was previously reported, we observed that the increased hydrophobicity of PMAA coatings from MAA resulted in excellent biocompatibility, demonstrated by growth and viability of dermal fibroblast cultures, making them prospective for biomedical applications. However, the LCST transition of these coatings did not induce temperature-controlled changes in protein (BSA) adsorption and cell (fibroblast) morphology.