Effect of tristetraprolin on esophageal squamous cell carcinoma cell proliferation

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY Tissue & cell Pub Date : 2025-06-01 Epub Date: 2025-02-07 DOI:10.1016/j.tice.2025.102785

Xiaoya Deng , Xiaoqin Luo , Zhanglan Fang , Xinyu Chen , Qinli Luo

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Abstract

Background

Tristetraprolin (TTP) can inhibit the abnormal proliferation of malignant tumors but there are no studies involving TTP and esophageal squamous cell carcinoma (ESCC). We aimed to determine the effect of TTP on ESCC cell proliferation and to elucidate the underlying mechanism.

Methods

The human ESCC cell line, KYSE-510, and the human ESCC cell line, KYSE-150, stably infected with tetracycline-inducible expression (Tet-on-TTP and Tet-on-EV, respectively) were screened with puromycin. After Tet-on-TTP KYSE-150 cells were treated with different concentrations of doxycycline [Dox] (0, 0.5, and 1 ug/mL), the levels of TTP mRNA and protein expression were detected by real-time ﬂuorescent quantitative PCR and western blotting, respectively. The effects of TTP on proliferation and migration were estimated by CCK-8 and Transwell assays, respectively. Cell apoptosis and cell cycle were measured by flow cytometry. Cellular apoptosis-related gene protein expression was determined by western blotting.

Results

TTP overexpression signiﬁcantly inhibited KYSE-510 and KYSE-150 proliferation. TTP overexpression also signiﬁcantly inhibited KYSE-150 migration. In addition, TTP expression upregulation promoted the KYSE-150 apoptosis and induced cell cycle arrest in the G₂ phase, downregulated Bcl-2 expression, and upregulated Bax expression.

Conclusion

TTP inhibited ESCC cell proliferation, promoted ESCC cell apoptosis, and arrested cell cycle progression in the G₂ phase.

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三曲丙林对食管鳞状细胞癌细胞增殖的影响

背景三曲普林（tristeprolin， TTP）可抑制恶性肿瘤的异常增殖，但TTP与食管鳞状细胞癌（ESCC）之间的关系尚未见相关研究。我们的目的是确定TTP对ESCC细胞增殖的影响，并阐明其潜在机制。方法用嘌呤霉素对稳定感染四环素诱导表达（分别为Tet-on-TTP和Tet-on-EV）的人ESCC细胞株KYSE-510和KYSE-150进行筛选。用不同浓度的多西环素[Dox]（0、0.5和1 ug/mL）处理Tet-on-TTP KYSE-150细胞后，分别采用实时荧光定量PCR和western blotting检测TTP mRNA和蛋白的表达水平。CCK-8法和Transwell法分别测定TTP对细胞增殖和迁移的影响。流式细胞术检测细胞凋亡和细胞周期。western blotting检测细胞凋亡相关基因蛋白表达。结果sttp过表达显著抑制KYSE-510和KYSE-150的增殖。TTP过表达也显著抑制了KYSE-150的迁移。此外，TTP表达上调可促进KYSE-150凋亡，诱导G2期细胞周期阻滞，下调Bcl-2表达，上调Bax表达。结论ttp抑制ESCC细胞增殖，促进ESCC细胞凋亡，阻滞细胞周期进展至G2期。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.