Unlocking the potential of Shewanella in metabolic engineering: Current status, challenges, and opportunities

IF 6.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic engineering Pub Date : 2025-05-01 Epub Date: 2025-02-12 DOI:10.1016/j.ymben.2025.02.002

Yi-Nan Liu , Zhourui Liu , Jian Liu , Yidan Hu , Bin Cao

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Abstract

Shewanella species are facultative anaerobes with distinctive electrochemical properties, making them valuable for applications in energy conversion and environmental bioremediation. Due to their well-characterized electron transfer mechanisms and ease of genetic manipulation, Shewanella spp. have emerged as a promising chassis for metabolic engineering. In this review, we provide a comprehensive overview of the advancements in Shewanella-based metabolic engineering. We begin by discussing the physiological characteristics of Shewanella, with a particular focus on its extracellular electron transfer (EET) capability. Next, we outline the use of Shewanella as a metabolic engineering chassis, presenting a general framework for strain construction based on the Design–Build–Test–Learn (DBTL) cycle and summarizing key advancements in the engineering of Shewanella's metabolic modules. Finally, we offer a perspective on the future development of Shewanella chassis, highlighting the need for deeper mechanistic insights, rational strain design, and interdisciplinary collaboration to drive further progress.

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释放希瓦氏菌在代谢工程中的潜力：现状、挑战和机遇。

希瓦氏菌是兼性厌氧菌，具有独特的电化学特性，在能量转化和环境生物修复方面具有重要的应用价值。由于其具有良好的电子传递机制和易于遗传操作，希瓦氏菌已成为代谢工程的有前途的基础。本文综述了基于舍瓦氏菌的代谢工程的研究进展。我们首先讨论希瓦氏菌的生理特性，特别关注其细胞外电子转移（EET）能力。接下来，我们概述了Shewanella作为代谢工程底盘的使用，提出了基于设计-构建-测试-学习（DBTL）周期的应变构建的一般框架，并总结了Shewanella代谢模块工程的关键进展。最后，我们对希瓦氏菌底盘的未来发展提出了展望，强调需要更深入的机械洞察、合理的应变设计和跨学科合作来推动进一步的发展。

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来源期刊

Metabolic engineering 工程技术-生物工程与应用微生物

CiteScore

15.60

自引率

6.00%

发文量

140

审稿时长

44 days

期刊介绍： Metabolic Engineering (MBE) is a journal that focuses on publishing original research papers on the directed modulation of metabolic pathways for metabolite overproduction or the enhancement of cellular properties. It welcomes papers that describe the engineering of native pathways and the synthesis of heterologous pathways to convert microorganisms into microbial cell factories. The journal covers experimental, computational, and modeling approaches for understanding metabolic pathways and manipulating them through genetic, media, or environmental means. Effective exploration of metabolic pathways necessitates the use of molecular biology and biochemistry methods, as well as engineering techniques for modeling and data analysis. MBE serves as a platform for interdisciplinary research in fields such as biochemistry, molecular biology, applied microbiology, cellular physiology, cellular nutrition in health and disease, and biochemical engineering. The journal publishes various types of papers, including original research papers and review papers. It is indexed and abstracted in databases such as Scopus, Embase, EMBiology, Current Contents - Life Sciences and Clinical Medicine, Science Citation Index, PubMed/Medline, CAS and Biotechnology Citation Index.