Unravelling the inhibition mechanism of 1-octene-3-ol combined with 3-heptene-2-one on Aspergillus flavus and its application in the preservation of wolfberries

IF 6.8 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2025-05-01 Epub Date: 2025-02-18 DOI:10.1016/j.postharvbio.2025.113462

Lijun Ling , Rongxiu Mo , Wenyue Zhang , Yijuan Jiang , Fanjin Kong , Lijun Feng , Yao Li , Rui Yue , Yongpeng Zhou

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Abstract

The antifungal activity of six microbial-derived volatile organic compounds (VOCs) [ 3-hepten-2-one (Hep), cis-3-octen-1-ol, 3-methylvalerate, isovalerate, 1-octen-3-ol (Oct), and 2-methylbutyrate] against Aspergillus flavus was investigated by determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). The results demonstrated that all 15 binary combinations of the VOCs presented additive effects against A. flavus. Among them, the combination of Hep and Oct, refers to C_OH, with concentrations of 2 μL L⁻¹ and 2.5 μL L⁻¹, respectively, displayed the most potent antifungal effect with the lowest concentration, in contrast to the individual MIC of Hep or Oct, which were reduced to 1/8 and 1/2. C_OH can induce membrane lipid peroxidation, enhance cell membrane permeability, and cause leakage of nucleic acids and proteins within the cell, and 1-octen-3-ol might play a leading role. Finally, applying C_OH at a concentration of 1/2 MIC_Oct + 1/4 MIC_Hep could effectively restrain the spread of A. flavus on wolfberries and exhibit excellent preservation effects. This study validates the antifungal effect of C_OH and may serve as a natural alternative to antimicrobial agents for post-harvest agricultural products.

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1-辛烯-3-醇联合3-庚烯-2- 1对黄曲霉的抑制机制及其在枸杞保鲜中的应用

通过测定最小抑菌浓度（MIC）和分数抑菌浓度指数（FICI），研究了6种微生物源性挥发性有机物（VOCs）[3-庚烯-2-酮（Hep）、顺式-3-辛烯-1-醇、3-甲基戊酸酯、异戊酸酯、1-辛烯-3-醇（Oct）和2-甲基丁酸酯]对黄曲霉的抑菌活性。结果表明，15种VOCs二元组合对黄曲霉均有加性作用。其中，Hep和Oct （COH）联合使用，浓度分别为2 μL L−1和2.5 μL L−1时，抑菌效果最好，浓度最低，而Hep和Oct单独使用的MIC分别为1/8和1/2。COH可诱导膜脂过氧化，增强细胞膜通透性，引起细胞内核酸和蛋白质的渗漏，其中1-辛烯-3-醇可能起主导作用。最后，1/2 MICOct + 1/4 MICHep浓度的COH可以有效抑制黄曲霉菌在枸杞上的传播，并具有良好的保存效果。该研究验证了COH的抗真菌作用，并可能作为收获后农产品抗菌药物的天然替代品。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.