Impact of time-restricted feeding on glycemic indices, vascular oxidative stress, and inflammation in an obese prediabetes rat model induced by a high-fat diet and sugar drink

IF 3.2 3区医学 Q2 NUTRITION & DIETETICS Nutrition Pub Date : 2025-01-23 DOI:10.1016/j.nut.2025.112692

Siti Qusyasyiah Ahmad Suhaimi , Ahmad Khusairi Azemi , Siti Safiah Mokhtar , Anani Aila Mat Zin , Aida Hanum Ghulam Rasool

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Abstract

Objective

This study aims to investigate the effects of time-restricted feeding (TRF) on glycaemic indices and aortic tissue oxidative stress and inflammation in an obese prediabetes rat model.

Methods and Procedures

Male Sprague-Dawley rats were divided into two normal and four obese groups. Obese prediabetes was induced by feeding a high-fat diet and sucrose water (HFSD) for 10 weeks; normal rats were given a standard diet and plain water. For the next 6 weeks, rats were grouped into the normal group (NR), which continued on the standard diet; the normal group was switched to TRF with the standard diet (NR + TRFSD); the prediabetes group (OR) was continued on HFSD; the prediabetes group was switched to TRF of HFSD (OR + TRFHFSD); the prediabetes group was switched to TRF of the standard diet (OR + TRFSD); and the prediabetes group was switched to the standard diet (OR + SD). Rats were then sacrificed, and aortic tissues were isolated and quantified for oxidative stress markers malondialdehyde, antioxidant enzyme superoxide dismutase, and inflammation markers tumor necrosis factor-α, and interleukin 1. Fasting blood glucose (FBG), body weight, Lee's index, serum insulin level, and resistance (Homeostatic Model Assessment of Insulin Resistance) were also measured.

Results

Mean FBG and body weight in obese groups were higher compared to the normal groups after 10 weeks of HFDSD. Both obese-prediabetes groups that underwent TRF had reduced levels of tumor necrosis factor-α, interleukin 1, body weight, Lee's index, FBG, and insulin resistance. Furthermore, obese prediabetes on TRF with SD also reduced levels of lipid peroxidation (malondialdehyde), insulin levels and increased levels of the antioxidant enzyme (superoxide dismutase).

Conclusion

TRF reduced weight, improved glycaemic indices, vascular oxidative stress, and inflammation in obese-prediabetic rats.

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来源期刊

Nutrition 医学-营养学

CiteScore

7.80

自引率

2.30%

发文量

300

审稿时长

60 days

期刊介绍： Nutrition has an open access mirror journal Nutrition: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Founded by Michael M. Meguid in the early 1980''s, Nutrition presents advances in nutrition research and science, informs its readers on new and advancing technologies and data in clinical nutrition practice, encourages the application of outcomes research and meta-analyses to problems in patient-related nutrition; and seeks to help clarify and set the research, policy and practice agenda for nutrition science to enhance human well-being in the years ahead.