Epidemiology, molecular characterization, and risk factors of Acanthamoeba spp., Blastocystis spp., and Cyclospora spp. infections in snakes in China

IF 2 2区农林科学 Q2 PARASITOLOGY Veterinary parasitology Pub Date : 2025-02-13 DOI:10.1016/j.vetpar.2025.110420

Yilei Zhang , Zhouchun Li , Xinyuan Wang, Kaili Gao, Lijie Tian, Olalekan Opeyemi Ayanniyi, Qianming Xu, Congshan Yang

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引用次数: 0

Abstract

Snakes are widely farmed in China for medicinal purposes and as pets worldwide. Acanthamoeba spp., Blastocystis spp., and Cyclospora spp. are significant zoonotic pathogens frequently discovered in various animals, causing diseases with global public health implications. However, their prevalence and zoonotic potential in snakes remain poorly understood. In this study, 812 snake faecal samples were collected across 28 China provinces. The partial small subunit (SSU) rRNA gene was amplified using polymerase chain reaction (PCR) to assess evolutionary relationships and genetic characterization. Detection rates for Acanthamoeba spp., Blastocystis spp., and Cyclospora spp. were 6.40 %, 3.33 %, and 2.71 %, respectively. Sequencing and phylogenetic analysis revealed that Cyclospora isolates were closely related to those found in humans and cattle. Subtyping for Blastocystis species identified two zoonotic subtypes (ST4, ST6) and four host-specific subtypes (ST10, ST15, ST21, ST42). Multiple Acanthamoeba genotypes were detected, including T4, T11, and T13. Furthermore, species, age, and living conditions are key risk factors. This study provides valuable insights into these infections in snakes and underscores the need for proper hygiene and One Health measures to reduce zoonotic transmission and environmental contamination.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.