Metabolomic Insights into the Promotional Effect of Mulberry Leaf Flavonoids on Monascus Fermentation for the Production of Pigments

IF 3.3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2025-02-18 DOI:10.1007/s12010-025-05183-7

Chun Liu, Xinyue Huang, Yanhao Huang, Biao Wang, Junqiang Jia, Qiongying Wu

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Abstract

In this study, adding mulberry leaf flavonoids (MLFs) during the liquid-state fermentation of Monascus purpureus significantly improved the production of Monascus pigments (MPs). Compared with the control group, the red, yellow, and orange pigment levels increased by 1.69, 1.4, and 1.29 times, respectively. Metabolomic analysis suggested adding rutin, the primary component of MLFs, induced changes in 32 significantly different metabolites. These changes included a significant increase in (S)-2,3,4,5-tetrahydropyridine-2-carboxylate, D-4′-phosphopantothenate, cis-4-hydroxy-D-proline, and L-isoleucine. Enriched Kyoto Encyclopedia of Genes and Genomics (KEGG) metabolic pathways revealed valine, leucine, and isoleucine biosynthesis, and phenylalanine metabolism improvements. These improvements help to activate the pigment synthesis pathway and promote pigment synthesis. These findings offer crucial insights into the impact of MLFs on Monascus purpureus metabolic pathways and suggest potential methods for increasing MP production.

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桑叶黄酮类化合物对红曲霉发酵生产色素的代谢组学研究。

在本研究中，在红曲霉液体发酵过程中添加桑叶黄酮（MLFs）可显著提高红曲霉色素（MPs）的产量。与对照组相比，红、黄、橙色素水平分别提高了1.69倍、1.4倍和1.29倍。代谢组学分析表明，加入主要成分芦丁可诱导32种不同代谢产物发生显著变化。这些变化包括(S)-2,3,4,5-四氢吡啶-2-羧酸盐、D-4'-磷酸前酸盐、顺式-4-羟基- d -脯氨酸和l -异亮氨酸的显著增加。丰富的京都基因与基因组学百科（KEGG）代谢途径揭示了缬氨酸、亮氨酸和异亮氨酸的生物合成，以及苯丙氨酸代谢的改善。这些改善有助于激活色素合成途径，促进色素合成。这些发现为mlf对红曲霉代谢途径的影响提供了重要的见解，并提出了增加MP产量的潜在方法。

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来源期刊

Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学

CiteScore

5.70

自引率

6.70%

发文量

460

审稿时长

5.3 months

期刊介绍： This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.