{"title":"Overexpression of a pathway-specific transcriptional factor CicD leads to the discovery of new phytotoxins in <i>Aspergillus nidulans</i>.","authors":"Xiaolin Liu, Jinda Hou, Lang Wang, Jing Yu, Xuewen Hou, Luning Zhou, Falei Zhang, Qian Che, Tianjiao Zhu, Dehai Li, Guojian Zhang","doi":"10.1039/d4ob02051k","DOIUrl":null,"url":null,"abstract":"<p><p>A NRPKS gene cluster <i>cic</i> in <i>Aspergillus nidulans</i> was activated by overexpression of the pathway-specific transcriptional factor CicD. Large-scale fermentation and chemical investigation of the engineered strain <i>A. nidulans</i> A1145/cicD led to the discovery of two new isoindolone-amino acid adducts, isoleucylcichorine (1) and leucylcichorine (2), along with three known derivatives. The structures of the new compounds were clearly determined by nuclear magnetic resonance (NMR) analysis, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) calculations. This study revealed that the overexpression of the pathway-specific transcriptional factor represents a promising approach for the discovery of new natural products in fungi.</p>","PeriodicalId":96,"journal":{"name":"Organic & Biomolecular Chemistry","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Organic & Biomolecular Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ob02051k","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ORGANIC","Score":null,"Total":0}
引用次数: 0
Abstract
A NRPKS gene cluster cic in Aspergillus nidulans was activated by overexpression of the pathway-specific transcriptional factor CicD. Large-scale fermentation and chemical investigation of the engineered strain A. nidulans A1145/cicD led to the discovery of two new isoindolone-amino acid adducts, isoleucylcichorine (1) and leucylcichorine (2), along with three known derivatives. The structures of the new compounds were clearly determined by nuclear magnetic resonance (NMR) analysis, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) calculations. This study revealed that the overexpression of the pathway-specific transcriptional factor represents a promising approach for the discovery of new natural products in fungi.
期刊介绍：
Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.
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