{"title":"Integrated Analysis of mRNAs, lncRNAs, and circRNAs Expression Profile in Human Colorectal Cancer.","authors":"Fei Tu, Mengfan Li, Lun Hai, Luyao Li, Tiesuo Zhao, Sheng Guo, Zhiwei Feng, Qingzhi Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer is the third most common cancer worldwide and one of the leading causes of cancer-related death. However, current treatment options are not ideal.</p><p><strong>Methods: </strong>In this study, mRNAs, lncRNAs, and circRNAs expression profiles in four pairs of HCT116 and FHC cells were detected by microarray technology. The potential functions and enriched pathways of the differentially expressed RNAs were predicted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses. Bioinformatics software was used to construct co-expression networks of lncRNA-miRNA-mRNA and circRNA-miRNA to reveal the potential mutual regulatory mechanisms of ceRNA.</p><p><strong>Results: </strong>The result showed that 6681 mRNAs, 12784 lncRNAs, and 14301 CircRNAs were changed in HCT116 and FHC groups. Meanwhile, the differentially expressed RNAs were confirmed by RT-qPCR and then focused on the up-regulated genes of hsa_circ_0025288, ENSG00000233429.9, and NT5E by Maximum difference expression level.</p><p><strong>Conclusion: </strong>This study provides a theoretical basis for the regulatory mechanism of non-coding RNAs in the pathogenesis and development of colorectal cancer cells. Therefore, this study pointed to ENSG00000233429.9 and hsa_circ_0025288 as potential molecular targets for colorectal cancer treatment in the future.</p>","PeriodicalId":7571,"journal":{"name":"Alternative therapies in health and medicine","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Alternative therapies in health and medicine","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Colorectal cancer is the third most common cancer worldwide and one of the leading causes of cancer-related death. However, current treatment options are not ideal.
Methods: In this study, mRNAs, lncRNAs, and circRNAs expression profiles in four pairs of HCT116 and FHC cells were detected by microarray technology. The potential functions and enriched pathways of the differentially expressed RNAs were predicted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses. Bioinformatics software was used to construct co-expression networks of lncRNA-miRNA-mRNA and circRNA-miRNA to reveal the potential mutual regulatory mechanisms of ceRNA.
Results: The result showed that 6681 mRNAs, 12784 lncRNAs, and 14301 CircRNAs were changed in HCT116 and FHC groups. Meanwhile, the differentially expressed RNAs were confirmed by RT-qPCR and then focused on the up-regulated genes of hsa_circ_0025288, ENSG00000233429.9, and NT5E by Maximum difference expression level.
Conclusion: This study provides a theoretical basis for the regulatory mechanism of non-coding RNAs in the pathogenesis and development of colorectal cancer cells. Therefore, this study pointed to ENSG00000233429.9 and hsa_circ_0025288 as potential molecular targets for colorectal cancer treatment in the future.
期刊介绍：
Launched in 1995, Alternative Therapies in Health and Medicine has a mission to promote the art and science of integrative medicine and a responsibility to improve public health. We strive to maintain the highest standards of ethical medical journalism independent of special interests that is timely, accurate, and a pleasure to read. We publish original, peer-reviewed scientific articles that provide health care providers with continuing education to promote health, prevent illness, and treat disease. Alternative Therapies in Health and Medicine was the first journal in this field to be indexed in the National Library of Medicine. In 2006, 2007, and 2008, ATHM had the highest impact factor ranking of any independently published peer-reviewed CAM journal in the United States—meaning that its research articles were cited more frequently than any other journal’s in the field.
Alternative Therapies in Health and Medicine does not endorse any particular system or method but promotes the evaluation and appropriate use of all effective therapeutic approaches. Each issue contains a variety of disciplined inquiry methods, from case reports to original scientific research to systematic reviews. The editors encourage the integration of evidence-based emerging therapies with conventional medical practices by licensed health care providers in a way that promotes a comprehensive approach to health care that is focused on wellness, prevention, and healing. Alternative Therapies in Health and Medicine hopes to inform all licensed health care practitioners about developments in fields other than their own and to foster an ongoing debate about the scientific, clinical, historical, legal, political, and cultural issues that affect all of health care.
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