Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum

Abstract

Strain HUAS MG91T, a novel actinobacterium, was isolated from the rhizosphere soil of Nicotiana tabacum collected from Changde City, China. This strain could form short, rod-shaped spores with a smooth surface and Rectiflexibles spore chains on Gause’s synthetic No.1 medium. Comparison of 16S rRNA gene sequences showed that strain HUAS MG91T exhibited the highest similarity to Streptomyces kunmingensis NBRC 14463 T (98.95%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain HUAS MG91T was closely related to S. kunmingensis NBRC 14463 T. However, the ANIm and dDDH values between them were 87.25% and 28.60%, respectively, lower than the 96.7% ANIm threshold and 70.0% cutoff point used as guideline values for Streptomyces species delineation. On the basis of phenotypic and genotypic differentiation from tested strains, strain HUAS MG91T is proposed to represent a novel species of the genus Streptomyces, named Streptomyces tabacisoli sp. nov. The type strain is HUAS MG91T (=MCCC 1K09288T = JCM 37027 T).