Natural Mackinawite-based Elimination of Vanadium and Ammonium from Wastewater in Autotrophic Biosystem

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Water Research Pub Date : 2025-02-19 DOI:10.1016/j.watres.2025.123333

Chenran Song, Song Wang, Qinghao Zhang, Min Li, Baogang Zhang

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Abstract

Vanadium (V) production results in significant amounts of wastewater, which often co-contains considerable ammonium (NH₄⁺) after being used as precipitants. Both pentavalent V [V(V)] and NH₄⁺ can be removed independently through biological process. However, internal interactive biotechnology for one-step elimination of V(V) and NH₄⁺ remains an enigma. In this study, we proposed biologically removing V(V) and NH₄⁺ simultaneously with natural mineral mackinawite as electron donor and its oxidation products as electron acceptors. Our bioreactor achieved a V(V) removal efficiency of 99.5 ± 0.22% and an NH₄⁺-N removal capacity of 49.5 ± 0.40 g/m³·d. V(V) was reduced to tetravalent V precipitates, while mackinawite was bio-oxidized to Fe(III) and sulfate. Metagenomic binning analysis indicated Sulfurivermis sp. mediated mackinawite oxidation and V(V) reduction. Putative Pseudomonas sp. conducted NH₄⁺ assimilation, anaerobic ammonium oxidation coupled to Fe(III) reduction (Feammox), and denitrification, achieving complete NH₄⁺-N removal. Real-time qPCR validated the upregulation of functional genes involved in V(V) reduction and nitrogen metabolisms, with improved functional enzyme activities. Cytochrome c, nicotinamide adenine dinucleotide, and extracellular polymeric substances promoted electron transfer, facilitating the elimination of both V(V) and NH₄⁺-N from wastewater. This study offers a novel and sustainable biological strategy for one-step treating V industrial wastewater.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.