Yang Zhou , Zuying Zhou , Lin Jin , Yue Peng , Jingwen Tang , Aimin Wang , Meng Zhou , Yueting Li , Lin Zheng , Yong Huang
{"title":"The effects and mechanisms of aqueous Persicaria capitata extract on uropathogenic Escherichia coli adhesion","authors":"Yang Zhou , Zuying Zhou , Lin Jin , Yue Peng , Jingwen Tang , Aimin Wang , Meng Zhou , Yueting Li , Lin Zheng , Yong Huang","doi":"10.1016/j.phymed.2025.156515","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Urinary tract infections (UTIs) in humans are common, with uropathogenic <em>Escherichia coli</em> (UPEC) being the primary pathogen. The adhesive capabilities of UPEC are a substantial pathogenicity factor. Due to limitations of first-line antibiotics, <em>Persicaria capitata</em> (Buch.-Ham. ex D. Don) H. Gross, a traditional Chinese medicinal plant, is frequently used to treat various urological disorders. However, its mechanism regarding bacterial adhesion, remain unclear.</div></div><div><h3>Purpose</h3><div>To investigate the effects and mechanisms of action of aqueous <em>P. capitata</em> extracts (PCE) on UPEC adhesion in T24 cells and rat models.</div></div><div><h3>Methods</h3><div>Broth microdilution and growth experiments were used to explore the direct antibacterial effects of PCE on UPEC. Additionally, motility assays were conducted. Different microscopy methods were used to further examine the mechanisms of action. Transcriptomic analysis and RT-qPCR were used to explore mechanisms on a molecular level. Relevant molecules were assessed using western blotting and immunohistochemistry.</div></div><div><h3>Results</h3><div>PCE modulated UPEC motility by disrupting the fimbriae and flagella. UPEC pathways, including those essential for constructing fimbriae and flagella, and bacterial motility, were affected. PCE reduced UPEC adhesion and invasion of T24 cells, altering the protein expression of adhesion-related molecules, by modulating the secretion of extracellular vesicles (EVs). It improved blood and urine parameters, reduced inflammatory markers, and ameliorated pathological changes in the kidneys and bladder of rats. Furthermore, the expression of adhesion-related molecules in bladder tissues decreased in the UTI rat model.</div></div><div><h3>Conclusions</h3><div>This study provides new insights into the mechanisms of herbal medicines in treating UTIs.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"139 ","pages":"Article 156515"},"PeriodicalIF":8.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytomedicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0944711325001564","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Urinary tract infections (UTIs) in humans are common, with uropathogenic Escherichia coli (UPEC) being the primary pathogen. The adhesive capabilities of UPEC are a substantial pathogenicity factor. Due to limitations of first-line antibiotics, Persicaria capitata (Buch.-Ham. ex D. Don) H. Gross, a traditional Chinese medicinal plant, is frequently used to treat various urological disorders. However, its mechanism regarding bacterial adhesion, remain unclear.
Purpose
To investigate the effects and mechanisms of action of aqueous P. capitata extracts (PCE) on UPEC adhesion in T24 cells and rat models.
Methods
Broth microdilution and growth experiments were used to explore the direct antibacterial effects of PCE on UPEC. Additionally, motility assays were conducted. Different microscopy methods were used to further examine the mechanisms of action. Transcriptomic analysis and RT-qPCR were used to explore mechanisms on a molecular level. Relevant molecules were assessed using western blotting and immunohistochemistry.
Results
PCE modulated UPEC motility by disrupting the fimbriae and flagella. UPEC pathways, including those essential for constructing fimbriae and flagella, and bacterial motility, were affected. PCE reduced UPEC adhesion and invasion of T24 cells, altering the protein expression of adhesion-related molecules, by modulating the secretion of extracellular vesicles (EVs). It improved blood and urine parameters, reduced inflammatory markers, and ameliorated pathological changes in the kidneys and bladder of rats. Furthermore, the expression of adhesion-related molecules in bladder tissues decreased in the UTI rat model.
Conclusions
This study provides new insights into the mechanisms of herbal medicines in treating UTIs.
期刊介绍：
Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
