Determination of Minimum Alveolar Concentrations of Isoflurane and Effective Plasma Concentration of Propofol in Common Marmosets (Callithrix jacchus)

IF 0.5 4区农林科学 Q3 VETERINARY SCIENCES Journal of Medical Primatology Pub Date : 2025-02-19 DOI:10.1111/jmp.70006

Kanako Muta, Makoto Koizumi, Takako Miyabe-Nishiwaki, Yusuke Sotomaru, Asako Nobukiyo, Hiroki Ohta, Hirotaka James Okano, Masatoshi Kamata, Dai Nagakubo, Ryohei Nishimura

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Abstract

Background

Common marmosets (Callithrix jacchus) have been widely used as laboratory animals; However, limited sedation and anesthetic protocols have yet to be established. In this study, the minimum alveolar concentration of an inhalant (isoflurane) and effective predicted plasma concentration of an injectable anesthetic (propofol) were measured for optimization of sedation and anesthetic protocols in marmosets.

Methods

The minimum alveolar concentrations (MACs) for several stimulations (nociceptive stimulation, endotracheal intubation, and non-painful direct stimulation), MAC-noci, MAC-extb, and MAC-awake, respectively, were measured for isoflurane with the up-and-down method from four healthy marmosets. Predicted plasma concentrations 50 (Cp50s), which are equivalent to MACs of isoflurane, Cp50-noci, Cp50-extb, and Cp50-awake, respectively, were measured for propofol.

Results

MAC-noci and MAC-extb of isoflurane in marmosets were 1.91% and 1.38%, respectively. MAC-awake was not determined owing to technical difficulties. Cp50-noci, Cp50-extb, and Cp50-awake were 9.45, 7.21, and 3.54 μg/mL, respectively.

Conclusions

The obtained results refined existing isoflurane and propofol for sedation and anesthesia in marmosets.

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猕猴异氟醚最低肺泡浓度和异丙酚有效血浆浓度的测定

普通狨猴（Callithrix jacchus）被广泛用作实验动物；然而，有限的镇静和麻醉方案尚未建立。在这项研究中，测量了吸入剂（异氟醚）的最低肺泡浓度和可注射麻醉剂（异丙酚）的有效预测血浆浓度，以优化狨猴的镇静和麻醉方案。方法采用升降法测定4只健康狨猴异氟醚在几种刺激（伤害性刺激、气管插管和非疼痛性直接刺激）、MAC-noci、MAC-extb和MAC-awake下的最低肺泡浓度（mac）。预测血浆浓度50 (cp50)，分别与异氟醚、Cp50-noci、Cp50-extb和Cp50-awake的MACs相当。结果异氟醚在狨猴体内的MAC-noci和mac - exb分别为1.91%和1.38%。由于技术上的困难，没有确定mac唤醒。Cp50-noci、Cp50-extb、Cp50-awake分别为9.45、7.21、3.54 μg/mL。结论所得结果完善了现有异氟醚和异丙酚在狨猴镇静麻醉中的应用。

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.