Deoxynivalenol modulated mucin expression and proinflammatory cytokine production, affecting susceptibility to enteroinvasive Escherichia coli infection in intestinal epithelial cells

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Journal of Food Science Pub Date : 2025-02-20 DOI:10.1111/1750-3841.70079

Murphy Lam Yim Wan, Vanessa Anna Co, Paul C Turner, Shah P Nagendra, Hani El-Nezami

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Abstract

Deoxynivalenol (DON) is a common mycotoxin in crops that could induce intestinal inflammation, affecting the susceptibility of intestinal epithelial cells (IECs) to pathogen infection. This study aimed to investigate DON's effects on mucin and cytokine production as part of the local immune system and how it affected intestinal susceptibility to pathogen infection. Caco-2 cells were exposed to DON followed by acute enteroinvasive Escherichia coli (EIEC) infection. An increase in EIEC attachment to DON-exposed cells was observed, probably in part, mediated by secretory MUC5AC mucins and membrane-bound MUC4 and MUC17 mucins. Additionally, DON with EIEC posttreatment led to significant changes in the gene expression of several proinflammatory cytokines (IL1α, IL1β, IL6, IL8, TNFα, and MCP-1), which may be in part, mediated by NK-κB and/or MAPK signaling pathways. These data suggested DON may exert immunomodulatory effects on IECs, altering the IEC susceptibility to bacterial infection.

Practical Application

The results suggested that DON might modulate immune responses by affecting mucus and cytokine production, which may affect the susceptibility of intestinal epithelial cells to pathogen infection.

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脱氧雪腐镰刀菌醇调节粘蛋白表达和促炎细胞因子的产生，影响肠上皮细胞对肠侵入性大肠杆菌感染的易感性

脱氧雪腐镰刀菌醇（DON）是作物中常见的真菌毒素，可诱导肠道炎症，影响肠上皮细胞（IECs）对病原体感染的易感性。本研究旨在探讨DON作为局部免疫系统的一部分对粘蛋白和细胞因子产生的影响，以及它如何影响肠道对病原体感染的易感性。Caco-2细胞暴露于DON后，急性肠侵袭性大肠杆菌（EIEC）感染。观察到暴露于don的细胞上的EIEC附着增加，可能部分是由分泌MUC5AC粘蛋白和膜结合MUC4和MUC17粘蛋白介导的。此外，DON与EIEC后处理导致几种促炎细胞因子（il -1 α、il -1 β、il - 6、il - 8、tnf - α和MCP-1）的基因表达发生显著变化，这可能部分由NK-κB和/或MAPK信号通路介导。这些数据提示DON可能对IECs发挥免疫调节作用，改变IECs对细菌感染的易感性。结果提示DON可能通过影响黏液和细胞因子的产生来调节免疫应答，从而影响肠上皮细胞对病原体感染的易感性。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.