First evidence of Tityus confluens Borelli, 1899 (Buthidae) venom altering purine metabolism in rat blood cells.

Abstract

Purinergic signaling pathways play crucial roles in regulating hemostatic and inflammatory responses, both of which are impacted by scorpion envenomation. Scorpion venoms are complex mixtures of various toxins, such as peptides, enzymes, and nucleotides. Previous research showed that the action of scorpion toxins on the purinergic system stems from their effects on purinergic receptors. Additionally, a study identified a putative ectonucleotidase in scorpion venom. This study aimed to investigate the ability of Tityus confluens venom (10, 50, and 100 µg/mL) to metabolize adenine nucleotides and its potential effects on purinergic enzyme activity in rat platelets and lymphocytes. The effects of T. confluens venom on E-NTPDase (ATP and ADP hydrolysis), E-5'-NT (AMP hydrolysis), and E-ADA (ADO hydrolysis) activities were analyzed. The results revealed that crude venom from T. confluens exhibited ATP hydrolysis activity at all tested concentrations. In lymphocytes, ADP hydrolysis was inhibited by 100 µg/mL crude venom, whereas ADO hydrolysis was increased by all venom concentrations. In platelets, ATP hydrolysis was inhibited by 50 and 100 µg/mL crude venom, whereas AMP and ADO hydrolysis were inhibited by all concentrations. When considered collectively, the data suggested an elevation in extracellular ATP levels and a reduction in extracellular ADO. These findings are in alignment with clinical manifestations of scorpion envenomation characterized by a pro-inflammatory milieu. Furthermore, this study demonstrated the intrinsic ATPase activity of T. confluens venom and its ability to modulate E-NTPDase, E-5'-NT, and E-ADA activities in rat blood cells.