{"title":"Replication kinetics of novel swine influenza A viruses: an approach to vaccine production.","authors":"Rodrigo Tapia, Rafael Medina, Víctor Neira","doi":"10.4206/ajvs.562.01","DOIUrl":null,"url":null,"abstract":"<p><p>Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of ≥128 HAU/50 μL with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine-producing laboratories.</p>","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":"56 2","pages":"85-90"},"PeriodicalIF":0.5000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845247/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Austral Journal of Veterinary Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4206/ajvs.562.01","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/22 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of ≥128 HAU/50 μL with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine-producing laboratories.
期刊介绍：
Austral Journal of Veterinary Sciences (formerly Archivos de Medicina Veterinaria) publishes original scientific contributions in English, containing the latest developments and discoveries in veterinary sciences. The journal covers topics such as animal health and production, preventive medicine, zoonosis, pharmacology and therapeutics, methods of diagnosis, and other areas related to the veterinary field.
Austral Journal of Veterinary Sciences aims to divulge information about advances in veterinary medicine among universities, research centres, industries, government agencies, biologists, agronomists and veterinarians.
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