G. López-Valencia, Katya Monserrat Meza-Silva, Ana Paulina Haro-Álvarez, Enrique Trasviña-Muñoz, Issa Carolina García-Reynoso, José Carlomán Herrera-Ramírez, Sergio Daniel Gómez-Gómez
Ehrlichia and Anaplasma are obligate intracellular, gram-negative bacteria with tropism for hematopoietic cells, especially leukocytes and platelets. There are several Ehrlichia species that infect dogs. Ehrlichia canis is transmitted by Rhipicephalus sanguineus and replicates within monocytes and macrophages, leading to canine monocytic ehrlichiosis, a disease of worldwide distribution. The clinical signs are varied and non-specific. Anaplasma has two species that infect dogs, Anaplasma phagocytophilum and Anaplasma platys, the second also transmitted by Rhipicephalus sanguineus. This study aimed to evaluate the epidemiology and hematologic changes associated with the presence of Ehrlichia spp. and Anaplasma spp. coinfection in dogs from Baja California. Complete hematological analysis, examination of buffy coat smears, and ELISA tests were performed on blood samples from three veterinary diagnostic laboratories from Mexicali and Tijuana cities in Baja California, Mexico. A total of 5,469 dog samples were analyzed. The overall prevalence of Ehrlichia spp., was 4.79%, with a distribution of 6.3% in Mexicali (OR: 2.39 CI: 1.69-3.17) and 2.5% in Tijuana. The peak of infection was found in September. Contact with other dogs and tick infestation were the risk factors associated with infection (P < 0.05). There was 96% co-infection only in Tijuana and 0% in Mexicali. Anemia, thrombocytopenia, and hyperproteinemia are associated with Ehrlichia spp., and Ehrlichia/Anaplasma infection. In view of the foregoing, we have to maintain epidemiologic vigilance, as well as look further into the ticks present in the state and the possibility of transmission of unusual pathogens.
{"title":"Prevalence, risk factors, and hematologic changes in dogs from Baja California with presence of Ehrlichia spp., and coinfection with Anaplasma spp.","authors":"G. López-Valencia, Katya Monserrat Meza-Silva, Ana Paulina Haro-Álvarez, Enrique Trasviña-Muñoz, Issa Carolina García-Reynoso, José Carlomán Herrera-Ramírez, Sergio Daniel Gómez-Gómez","doi":"10.4206/ajvs.562.06","DOIUrl":"https://doi.org/10.4206/ajvs.562.06","url":null,"abstract":"Ehrlichia and Anaplasma are obligate intracellular, gram-negative bacteria with tropism for hematopoietic cells, especially leukocytes and platelets. There are several Ehrlichia species that infect dogs. Ehrlichia canis is transmitted by Rhipicephalus sanguineus and replicates within monocytes and macrophages, leading to canine monocytic ehrlichiosis, a disease of worldwide distribution. The clinical signs are varied and non-specific. Anaplasma has two species that infect dogs, Anaplasma phagocytophilum and Anaplasma platys, the second also transmitted by Rhipicephalus sanguineus. This study aimed to evaluate the epidemiology and hematologic changes associated with the presence of Ehrlichia spp. and Anaplasma spp. coinfection in dogs from Baja California. Complete hematological analysis, examination of buffy coat smears, and ELISA tests were performed on blood samples from three veterinary diagnostic laboratories from Mexicali and Tijuana cities in Baja California, Mexico. A total of 5,469 dog samples were analyzed. The overall prevalence of Ehrlichia spp., was 4.79%, with a distribution of 6.3% in Mexicali (OR: 2.39 CI: 1.69-3.17) and 2.5% in Tijuana. The peak of infection was found in September. Contact with other dogs and tick infestation were the risk factors associated with infection (P < 0.05). There was 96% co-infection only in Tijuana and 0% in Mexicali. Anemia, thrombocytopenia, and hyperproteinemia are associated with Ehrlichia spp., and Ehrlichia/Anaplasma infection. In view of the foregoing, we have to maintain epidemiologic vigilance, as well as look further into the ticks present in the state and the possibility of transmission of unusual pathogens.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141663658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sustainable livestock production is essential to ensure the availability of food and resources, and to address the social, economic, and environmental challenges that threaten conventional livestock production. While there is consensus among economic, social, and scientific groups on the need to assess sustainability to make decisions that protect resources for present and future generations, there are few sustainability assessment tools that address it holistically. The aim of this study was to develop an assessment tool applicable to farms by identifying the indicators currently applied in dairy farms, based on a systematic literature review and expert opinion. This study used the Delphi methodology to explore sustainability indicators at the farm level. A panel of seven expert researchers and academics in livestock sustainability and animal welfare participated in the study. A high level of consensus was found for 15 economic indicators, 14 social indicators, 20 environmental indicators, and 16 animal welfare indicators. Some indicators, such as financial autonomy, transmissibility, cow and labor productivity, husbandry system, labor intensity, community bonding, labor satisfaction, biodiversity, crop rotation, fertilization, manure management, and water management, showed a high level of consensus and were considered useful in assessing sustainability on dairy farms. In addition, livestock sustainability experts reached a high consensus on 16 animal welfare indicators that could be useful in assessing farm sustainability. These results provide a solid basis for sustainability indicators in the economic, social, environmental, and animal welfare dimensions, which could serve as a basis for developing a sustainability assessment tool for dairy farms.
{"title":"Use of Delphi methodology to select sustainability indicators on dairy farms: an exploration of environmental, economic, social and animal welfare dimensions","authors":"Melissa Sánchez-Hidalgo, Tamara Tadich","doi":"10.4206/ajvs.562.05","DOIUrl":"https://doi.org/10.4206/ajvs.562.05","url":null,"abstract":"Sustainable livestock production is essential to ensure the availability of food and resources, and to address the social, economic, and environmental challenges that threaten conventional livestock production. While there is consensus among economic, social, and scientific groups on the need to assess sustainability to make decisions that protect resources for present and future generations, there are few sustainability assessment tools that address it holistically. The aim of this study was to develop an assessment tool applicable to farms by identifying the indicators currently applied in dairy farms, based on a systematic literature review and expert opinion. This study used the Delphi methodology to explore sustainability indicators at the farm level. A panel of seven expert researchers and academics in livestock sustainability and animal welfare participated in the study. A high level of consensus was found for 15 economic indicators, 14 social indicators, 20 environmental indicators, and 16 animal welfare indicators. Some indicators, such as financial autonomy, transmissibility, cow and labor productivity, husbandry system, labor intensity, community bonding, labor satisfaction, biodiversity, crop rotation, fertilization, manure management, and water management, showed a high level of consensus and were considered useful in assessing sustainability on dairy farms. In addition, livestock sustainability experts reached a high consensus on 16 animal welfare indicators that could be useful in assessing farm sustainability. These results provide a solid basis for sustainability indicators in the economic, social, environmental, and animal welfare dimensions, which could serve as a basis for developing a sustainability assessment tool for dairy farms.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141680557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leandro Cádiz, Fernando Navarrete, Paulina Torres, Hector Hidalgo
Species of the Pasteurella genus are part of the oropharyngeal microbiota of many animal species. In poultry, Pasteurella multocida causes fowl cholera, the chronic manifestation of which can include neurological symptoms. Pasteurella canis causes osteomyelitis and other infections in humans. To the best of our knowledge, this is the first report of cranial osteomyelitis associated with Pasteurella canis in broiler chickens in Chile.
{"title":"Cranial osteomyelitis associated with Pasteurella canis in broiler chickens","authors":"Leandro Cádiz, Fernando Navarrete, Paulina Torres, Hector Hidalgo","doi":"10.4206/ajvs.562.04","DOIUrl":"https://doi.org/10.4206/ajvs.562.04","url":null,"abstract":"Species of the Pasteurella genus are part of the oropharyngeal microbiota of many animal species. In poultry, Pasteurella multocida causes fowl cholera, the chronic manifestation of which can include neurological symptoms. Pasteurella canis causes osteomyelitis and other infections in humans. To the best of our knowledge, this is the first report of cranial osteomyelitis associated with Pasteurella canis in broiler chickens in Chile.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. R. Vazquez Avendaño, César Cortez Romero, Demetrio Alonso Ambríz García, María de los Angeles Fortis Barrera, Mara Patricia Chávez Ortega, Hilda Loza Rodríguez, Gerardo Blancas Flores, María del Carmen Navarro Maldonado
Oviductal (OF) and uterine (UF) fluids are a complex mixture of ions and macromolecules dissolved in water, derived from the secretions of secretory cells and transudates of the circulatory system. Through proteomics, OF and UF have been analyzed in different domestic species throughout the estrous cycle or during the first days of pregnancy. Therefore, the aim of this study was to evaluate the volume, osmolarity, concentration and distribution pattern of proteins, as well as the identification of OVGP1, HSP70 and ezrin proteins for their importance in reproductive physiology, in OF and UF from adult criollo type domestic sheep during the early luteal phase of the estrous cycle. An average of 3.2±1.5 uL OF and 17±0.5 uL UF per reproductive system were obtained; osmolarity was 343±20.8 mOsm kg-1 and 280±96.2 mOsm kg-1 and protein concentration was 71.9±23.8 g L-1 and 21.8±1.1 g L-1, respectively. In the protein distribution pattern, 20 bands were observed in the OF and 14 bands in the UF. Of these, 14 and 8 were specific for OF and UF, respectively, and 6 were common for both. The spectra of the protein molecular weights were 24–324 and 29–353 kDa for OF and UF, respectively. The presence of OVPG1, HSP70 and ezrin proteins in both fluids was identified, being in greater quantity in the OF (P < 0.0005). The volume recovered from the UF was five times greater than that of the OF. Both osmolarity and protein concentration were higher in OF than in UF (1.2 and 3 times higher). The pattern of protein distribution between the OF and UF was different, being more complex in the OF. OVGP1, HSP70 and ezrin were identified in the OF and UF, and were found in greater quantities in the OF.
输卵管液（OF）和子宫液（UF）是溶于水的离子和大分子的复杂混合物，来自分泌细胞的分泌物和循环系统的渗出物。通过蛋白质组学，对不同家畜在整个发情周期或怀孕初期的 OF 和 UF 进行了分析。因此，本研究的目的是评估发情周期早期黄体期成年克里奥罗型家羊 OF 和 UF 中蛋白质的体积、渗透压、浓度和分布模式，并鉴定 OVGP1、HSP70 和 ezrin 蛋白，以确定它们在生殖生理中的重要性。每个生殖系统平均获得 3.2±1.5 uL OF 和 17±0.5 uL UF；渗透压分别为 343±20.8 mOsm kg-1 和 280±96.2 mOsm kg-1，蛋白质浓度分别为 71.9±23.8 g L-1 和 21.8±1.1 g L-1。在蛋白质分布图中，OF 中观察到 20 条带，UF 中观察到 14 条带。其中，14 条和 8 条分别为 OF 和 UF 所特有，6 条为两者所共有。在光谱中，OF 和 UF 的蛋白质分子量分别为 24-324 kDa 和 29-353 kDa。在两种液体中都发现了 OVPG1、HSP70 和 ezrin 蛋白，其中 OF 的含量更高（P < 0.0005）。UF 的回收体积是 OF 的五倍。OF 中的渗透压和蛋白质浓度均高于 UF（分别高出 1.2 倍和 3 倍）。蛋白在 OF 和 UF 中的分布模式不同，在 OF 中更为复杂。在 OF 和 UF 中发现了 OVGP1、HSP70 和 ezrin，在 OF 中发现的数量更多。
{"title":"Physicochemical characteristics and protein profile of oviductal and uterine fluids from domestic sheep","authors":"J. R. Vazquez Avendaño, César Cortez Romero, Demetrio Alonso Ambríz García, María de los Angeles Fortis Barrera, Mara Patricia Chávez Ortega, Hilda Loza Rodríguez, Gerardo Blancas Flores, María del Carmen Navarro Maldonado","doi":"10.4206/ajvs.562.03","DOIUrl":"https://doi.org/10.4206/ajvs.562.03","url":null,"abstract":"Oviductal (OF) and uterine (UF) fluids are a complex mixture of ions and macromolecules dissolved in water, derived from the secretions of secretory cells and transudates of the circulatory system. Through proteomics, OF and UF have been analyzed in different domestic species throughout the estrous cycle or during the first days of pregnancy. Therefore, the aim of this study was to evaluate the volume, osmolarity, concentration and distribution pattern of proteins, as well as the identification of OVGP1, HSP70 and ezrin proteins for their importance in reproductive physiology, in OF and UF from adult criollo type domestic sheep during the early luteal phase of the estrous cycle. An average of 3.2±1.5 uL OF and 17±0.5 uL UF per reproductive system were obtained; osmolarity was 343±20.8 mOsm kg-1 and 280±96.2 mOsm kg-1 and protein concentration was 71.9±23.8 g L-1 and 21.8±1.1 g L-1, respectively. In the protein distribution pattern, 20 bands were observed in the OF and 14 bands in the UF. Of these, 14 and 8 were specific for OF and UF, respectively, and 6 were common for both. The spectra of the protein molecular weights were 24–324 and 29–353 kDa for OF and UF, respectively. The presence of OVPG1, HSP70 and ezrin proteins in both fluids was identified, being in greater quantity in the OF (P < 0.0005). The volume recovered from the UF was five times greater than that of the OF. Both osmolarity and protein concentration were higher in OF than in UF (1.2 and 3 times higher). The pattern of protein distribution between the OF and UF was different, being more complex in the OF. OVGP1, HSP70 and ezrin were identified in the OF and UF, and were found in greater quantities in the OF.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141346670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. D. Rodríguez-Cortez, Cecilia Montoya-Carrillo, H. D. Nájera-Rivera, Alejandro Zaldivar-Gómez, Enrique Herrera López, Oscar Rico-Chavez, H. Ramírez Álvarez, A. De Las Peñas, E. Díaz-Aparicio, Laura Cobos-Marín
Mexican goat production systems face infection risks from Brucella melitensis, small ruminant lentivirus (SRLv) and Mycobacterium avium subsp. paratuberculosis (MAP); agents that cause great economic losses and directly affect public health (brucellosis and paratuberculosis [PTb]). Currently, there are no diagnostic tests applicable at large scale nor epidemiological information regarding the seroprevalence for these infectious diseases. For this study, a multiplex antibody assay (Luminex®) was used to retrospectively study the seroprevalence of brucellosis, SRLv, and PTb in the sera of 983 goats from nine Mexican states. Sera were obtained between 2014 and 2019. Antibodies against all three infectious diseases were detected in almost all tested samples. The estimated seroprevalence values ranged from 37% to 78% for brucellosis, 21% to 65% for SRLv, and 0% to 13% for PTb. The multiplex assay (Luminex®) is a simple, accessible, efficient, and cost-effective seroprevalence monitoring tool for brucellosis, SRLv, and PTb, and can be used as a large-scale approach.
{"title":"Retrospective seroepidemiological study of small ruminant lentivirus, paratuberculosis and brucellosis in goats from Mexico, based on multiplex assay","authors":"A. D. Rodríguez-Cortez, Cecilia Montoya-Carrillo, H. D. Nájera-Rivera, Alejandro Zaldivar-Gómez, Enrique Herrera López, Oscar Rico-Chavez, H. Ramírez Álvarez, A. De Las Peñas, E. Díaz-Aparicio, Laura Cobos-Marín","doi":"10.4206/ajvs.562.02","DOIUrl":"https://doi.org/10.4206/ajvs.562.02","url":null,"abstract":"Mexican goat production systems face infection risks from Brucella melitensis, small ruminant lentivirus (SRLv) and Mycobacterium avium subsp. paratuberculosis (MAP); agents that cause great economic losses and directly affect public health (brucellosis and paratuberculosis [PTb]). Currently, there are no diagnostic tests applicable at large scale nor epidemiological information regarding the seroprevalence for these infectious diseases. For this study, a multiplex antibody assay (Luminex®) was used to retrospectively study the seroprevalence of brucellosis, SRLv, and PTb in the sera of 983 goats from nine Mexican states. Sera were obtained between 2014 and 2019. Antibodies against all three infectious diseases were detected in almost all tested samples. The estimated seroprevalence values ranged from 37% to 78% for brucellosis, 21% to 65% for SRLv, and 0% to 13% for PTb. The multiplex assay (Luminex®) is a simple, accessible, efficient, and cost-effective seroprevalence monitoring tool for brucellosis, SRLv, and PTb, and can be used as a large-scale approach.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141364524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jose Dellis Rocha, Catherine Opitz, Vicente Cárdenas, Cristal Mella, Daniel Medina
Water bodies constitute natural, social, and ecological heritage under constant threat from the footprint of human action. They are the habitats of many species and play a key role in sustaining biodiversity in different ecosystems. However, anthropogenic activity can result in eutrophication of water bodies, favoring the excessive growth of microorganisms, which can be a risk factor for animal, human, and environmental health. The Agricultural and Livestock Service [Servicio Agrícola y Ganadero, (SAG)] of Chile developed a list of Mandatory Reportable Diseases (MRD) to report suspected contagious diseases of veterinary relevance. In this sense, the detection of microorganisms in water bodies for the characterization of their sanitary status is important for the development of monitoring strategies and the prevention of possible diseases. This study aimed to determine the presence of potentially harmful bacteria in Llanquihue urban wetlands. Here, we used metagenomic sequencing of amplicons to characterize the composition of microorganisms in three urban wetlands in the city of Llanquihue, which could be a risk factor for animal health. Our main finding was the detection of genera that may be associated with MRD, in addition to other microbial genera that have pathogenic potential. On the other hand, we also report the presence of cyanobacteria genera that can be involved in toxin production in water bodies as another potential source of risk. This is the first study to use massive sequencing techniques to analyze the sanitary status of urban wetlands in the city of Llanquihue.
水体是自然、社会和生态遗产，不断受到人类活动足迹的威胁。水体是许多物种的栖息地，在维持不同生态系统的生物多样性方面发挥着关键作用。然而，人类活动会导致水体富营养化，有利于微生物的过度生长，从而成为动物、人类和环境健康的危险因素。智利农业和畜牧业服务局（Servicio Agrícola y Ganadero，SAG）制定了一份强制报告疾病（MRD）清单，用于报告兽医相关的疑似传染病。从这个意义上说，检测水体中的微生物以确定其卫生状况，对于制定监测战略和预防可能发生的疾病非常重要。本研究旨在确定兰基韦城市湿地中是否存在潜在的有害细菌。在这里，我们使用元基因组测序扩增子来描述兰基胡埃市三个城市湿地中微生物组成的特征，这些微生物可能是动物健康的风险因素。我们的主要发现是发现了可能与 MRD 有关的菌属，以及其他具有致病潜力的微生物菌属。另一方面，我们还发现水体中存在可能参与毒素产生的蓝藻属，这也是另一个潜在的风险来源。这是首次使用大规模测序技术分析兰基韦市城市湿地卫生状况的研究。
{"title":"Identification of potentially harmful bacterial genera of veterinary relevance in the Llanquihue urban wetlands","authors":"Jose Dellis Rocha, Catherine Opitz, Vicente Cárdenas, Cristal Mella, Daniel Medina","doi":"10.4206/ajvs.561.06","DOIUrl":"https://doi.org/10.4206/ajvs.561.06","url":null,"abstract":"Water bodies constitute natural, social, and ecological heritage under constant threat from the footprint of human action. They are the habitats of many species and play a key role in sustaining biodiversity in different ecosystems. However, anthropogenic activity can result in eutrophication of water bodies, favoring the excessive growth of microorganisms, which can be a risk factor for animal, human, and environmental health. The Agricultural and Livestock Service [Servicio Agrícola y Ganadero, (SAG)] of Chile developed a list of Mandatory Reportable Diseases (MRD) to report suspected contagious diseases of veterinary relevance. In this sense, the detection of microorganisms in water bodies for the characterization of their sanitary status is important for the development of monitoring strategies and the prevention of possible diseases. This study aimed to determine the presence of potentially harmful bacteria in Llanquihue urban wetlands. Here, we used metagenomic sequencing of amplicons to characterize the composition of microorganisms in three urban wetlands in the city of Llanquihue, which could be a risk factor for animal health. Our main finding was the detection of genera that may be associated with MRD, in addition to other microbial genera that have pathogenic potential. On the other hand, we also report the presence of cyanobacteria genera that can be involved in toxin production in water bodies as another potential source of risk. This is the first study to use massive sequencing techniques to analyze the sanitary status of urban wetlands in the city of Llanquihue.\u0000 ","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139596075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leonor Miranda Jiménez, María Monserrat López Velázquez, Adrián Raymundo Quero Carrillo, Alejandrina Robledo Paz
Selenium plays an important role in bodily functions. It activates immune cells such as neutrophils and immunity through T cells. However, its role in ovarian follicular gene expression has not been reported. The effects of selenomethionine (SeMet) on gene expression in in vitro ewe follicles and their relationship with immunity and apoptosis were studied. Preovulatory follicles were randomly cultured, and SeMet was added to the culture, followed by incubation for 24 h. Total RNA was extracted from follicles and placed on a microarray chip. Gene expression was analyzed using GenArise and classified using the DAVID v6.8 bioinformatics program based on the Gene Ontology and Kyoto Encyclopedia for Genes and Genomes (KEGG). Gene pathways were designed using Cytoscape v3.7.2. There were 2,538 differentially expressed genes (DEGs): 1,228 upregulated and 1,310 downregulated. The upregulated genes were classified into 32, 18, and 12 nodes related to biological processes, cellular components, and molecular functions, respectively. KEGG assigned these genes to 41 metabolic and signaling pathways. 94 genes were involved in eight pathways associated with immune processes. The expression levels of CD8, NFAT2, and CD48 were quantified using RT-qPCR. Selenium activated gene expression in the in vitro preovulatory follicles of ewes, some of which were related to the immune system. The relationship among CD8, NFAT2, and CD48 genes suggests a possible immune pathway related to follicular apoptosis regulation.
{"title":"Selenium affects genes associated with immunity and apoptosis in in vitro follicles of ewes","authors":"Leonor Miranda Jiménez, María Monserrat López Velázquez, Adrián Raymundo Quero Carrillo, Alejandrina Robledo Paz","doi":"10.4206/ajvs.561.05","DOIUrl":"https://doi.org/10.4206/ajvs.561.05","url":null,"abstract":"Selenium plays an important role in bodily functions. It activates immune cells such as neutrophils and immunity through T cells. However, its role in ovarian follicular gene expression has not been reported. The effects of selenomethionine (SeMet) on gene expression in in vitro ewe follicles and their relationship with immunity and apoptosis were studied. Preovulatory follicles were randomly cultured, and SeMet was added to the culture, followed by incubation for 24 h. Total RNA was extracted from follicles and placed on a microarray chip. Gene expression was analyzed using GenArise and classified using the DAVID v6.8 bioinformatics program based on the Gene Ontology and Kyoto Encyclopedia for Genes and Genomes (KEGG). Gene pathways were designed using Cytoscape v3.7.2. There were 2,538 differentially expressed genes (DEGs): 1,228 upregulated and 1,310 downregulated. The upregulated genes were classified into 32, 18, and 12 nodes related to biological processes, cellular components, and molecular functions, respectively. KEGG assigned these genes to 41 metabolic and signaling pathways. 94 genes were involved in eight pathways associated with immune processes. The expression levels of CD8, NFAT2, and CD48 were quantified using RT-qPCR. Selenium activated gene expression in the in vitro preovulatory follicles of ewes, some of which were related to the immune system. The relationship among CD8, NFAT2, and CD48 genes suggests a possible immune pathway related to follicular apoptosis regulation.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139608176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marwa Yehia, A. Gamal, F. I. Abo El-Ela, A. S. Abdel-Baki, Samar M Ibrahium, Khaled AM Shokier, S. Al-Quraishy, Ahmed O Hassan, Noha H Abdelgelil, S. Aboelhadid
The current study aimed to evaluate the antimicrobial activity of carvacrol-loaded invasomes (CLI) against multidrug-resistant (MDR) Enterobacteriaceae and its mechanical vector, the housefly. CLI were prepared and characterized in the laboratory. Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Enteritidis, Salmonella enterica subsp. enterica serovar Typhimurium, and Klebsiella oxytoca were among the MDR enterobacteriaceae stains investigated. These strains were first isolated and identified from naturally infected chickens. The antibacterial activity of CLI against the MDR isolates was evaluated using the diffusion method. In addition, the insecticidal activity of CLI against housefly larvae and pupae was tested. The MDR index of all evaluated isolates was greater than 20%, indicating that they were all multidrug-resistant. CLI decreased the growth of all isolates except S. Typhimurium and P. aeruginosa at a dose of 0.0125%; however, pure carvacrol inhibited the growth of only Klebsiella oxytoca. Furthermore, both CLI and pure carvacrol inhibited Klebsiella oxytoca growth at different concentrations. CLI inhibited E. coli and S. enteritidis at lower concentrations than pure carvacrol, even at a doubled concentration. Carvacrol and CLI caused significant larval mortality even at low concentrations, with LC50 reached at concentartions of 2.54 and 2.19 µl/ml, respectively. Furthermore, at a low concentration of 3.125 µl/ml, both elicited a high percentage inhibition rate (PIR) in pupae. In conclusion, CLI demonstrated substantial antibacterial action, particularly against MDR isolates, as well as pesticide activity against houseflies.
{"title":"Carvacrol-loaded invasomes efficacy against multidrug resistant isolates of Enterobacteriaceae and housefly","authors":"Marwa Yehia, A. Gamal, F. I. Abo El-Ela, A. S. Abdel-Baki, Samar M Ibrahium, Khaled AM Shokier, S. Al-Quraishy, Ahmed O Hassan, Noha H Abdelgelil, S. Aboelhadid","doi":"10.4206/ajvs.561.04","DOIUrl":"https://doi.org/10.4206/ajvs.561.04","url":null,"abstract":"The current study aimed to evaluate the antimicrobial activity of carvacrol-loaded invasomes (CLI) against multidrug-resistant (MDR) Enterobacteriaceae and its mechanical vector, the housefly. CLI were prepared and characterized in the laboratory. Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Enteritidis, Salmonella enterica subsp. enterica serovar Typhimurium, and Klebsiella oxytoca were among the MDR enterobacteriaceae stains investigated. These strains were first isolated and identified from naturally infected chickens. The antibacterial activity of CLI against the MDR isolates was evaluated using the diffusion method. In addition, the insecticidal activity of CLI against housefly larvae and pupae was tested. The MDR index of all evaluated isolates was greater than 20%, indicating that they were all multidrug-resistant. CLI decreased the growth of all isolates except S. Typhimurium and P. aeruginosa at a dose of 0.0125%; however, pure carvacrol inhibited the growth of only Klebsiella oxytoca. Furthermore, both CLI and pure carvacrol inhibited Klebsiella oxytoca growth at different concentrations. CLI inhibited E. coli and S. enteritidis at lower concentrations than pure carvacrol, even at a doubled concentration. Carvacrol and CLI caused significant larval mortality even at low concentrations, with LC50 reached at concentartions of 2.54 and 2.19 µl/ml, respectively. Furthermore, at a low concentration of 3.125 µl/ml, both elicited a high percentage inhibition rate (PIR) in pupae. In conclusion, CLI demonstrated substantial antibacterial action, particularly against MDR isolates, as well as pesticide activity against houseflies.\u0000 ","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139618027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viviana M Bravo, Gerardo Acosta-Jamett, Carmen Gallo
In Chile, selling cattle through auction markets is common, and involves various stressful events. The objective of this study was to determine the effects of selling weaned beef calves through an auction market on indicators of physiological stress. Considering that transportation is invariably linked to any commercialization procedure, we compared the effects of exposing calves to transport and sale through an auction market versus transporting them directly between farms. Twenty calves of 175 (+/-37.5) kg live weight were transported from their origin farm to an auction market (2.5 h journey), where 10 calves were unloaded and exposed to regular handling within the market (unloading, sorting, regrouping, penning, sale yard showing) and thereafter loaded and transported back to the farm (TM). The remaining 10 calves were not unloaded at the market and were immediately transported back to the farm, simulating a direct sale (TD). Data collection (blood samples, body temperature, and live weight) was performed at the farm in both groups before loading (initial, 0 h), at the end of the process, after unloading TM calves back on the farm (after unloading, 12 h), and during recovery at 24, 48, and 72 h after the initial sampling. We used mixed-effects models to compare treatments and samplings. No differences were found between treatments in terms of live weight loss, maximum eye temperature, plasma beta-hydroxybutyrate, and serum cortisol concentrations. The rectal temperature was higher (p < 0.05) in TM after treatment and remained higher (p > 0.05) in the samples than in TD. Calves that passed through the market showed higher (p < 0.05) plasma CK activity after unloading (12 h) than did those that did not. The mean glucose concentration increased (p < 0.05) at 12h and remained higher than the initial value (0 h) in both the calf groups. This was a preliminary study, and according to the results, experiments should be repeated to test more animals under different situations and premises.
{"title":"Effects of selling through an auction market on physiological variables in beef calves","authors":"Viviana M Bravo, Gerardo Acosta-Jamett, Carmen Gallo","doi":"10.4206/ajvs.561.03","DOIUrl":"https://doi.org/10.4206/ajvs.561.03","url":null,"abstract":"In Chile, selling cattle through auction markets is common, and involves various stressful events. The objective of this study was to determine the effects of selling weaned beef calves through an auction market on indicators of physiological stress. Considering that transportation is invariably linked to any commercialization procedure, we compared the effects of exposing calves to transport and sale through an auction market versus transporting them directly between farms. Twenty calves of 175 (+/-37.5) kg live weight were transported from their origin farm to an auction market (2.5 h journey), where 10 calves were unloaded and exposed to regular handling within the market (unloading, sorting, regrouping, penning, sale yard showing) and thereafter loaded and transported back to the farm (TM). The remaining 10 calves were not unloaded at the market and were immediately transported back to the farm, simulating a direct sale (TD). Data collection (blood samples, body temperature, and live weight) was performed at the farm in both groups before loading (initial, 0 h), at the end of the process, after unloading TM calves back on the farm (after unloading, 12 h), and during recovery at 24, 48, and 72 h after the initial sampling. We used mixed-effects models to compare treatments and samplings. No differences were found between treatments in terms of live weight loss, maximum eye temperature, plasma beta-hydroxybutyrate, and serum cortisol concentrations. The rectal temperature was higher (p < 0.05) in TM after treatment and remained higher (p > 0.05) in the samples than in TD. Calves that passed through the market showed higher (p < 0.05) plasma CK activity after unloading (12 h) than did those that did not. The mean glucose concentration increased (p < 0.05) at 12h and remained higher than the initial value (0 h) in both the calf groups. This was a preliminary study, and according to the results, experiments should be repeated to test more animals under different situations and premises.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathalia M Correa Valencia, José Miguel Hernández Agudelo, Jorge A Fernández Silva
Bacteriological culture of Mycobacterium avium subsp. paratuberculosis (MAP) is considered the gold standard to confirm its presence in several matrices for Johne’s disease diagnosis. Whether it is a liquid or solid culture, a problem with MAP culture is that non-interpretable results arise because of overgrowth by other microorganisms, making MAP growth and identification more difficult or impossible. We systematically reviewed published decontamination protocols and their effects on MAP culture from bovine fecal and environmental samples on solid media. Based on our findings, we suggest a step-by-step decontamination protocol. The OVID®/MEDLINE, PubMed®, SciELO Citation Index®, and Redalyc® platforms as well as the International Colloquium on Paratuberculosis (ICP) proceedings and the reference lists were reviewed to identify relevant studies. The inclusion criteria considered articles published in English, Portuguese, French, German, Spanish, and peer-reviewed journals. The exclusion criteria included unrelated topics, species other than bovines, other than environmental/faecal samples, other than diagnostic techniques of interest, and non-original articles. Definitive studies were obtained through the authors’ consensus regarding their eligibility and quality. In total, 1,004 publications matched the search terms, and 27 articles met the inclusion criteria, of which 45 derived and reported 15 different decontamination protocols. The centrifugation-one-step hexadecylpyridinium chloride protocol, which used over 22,154 fecal samples in three studies, was found to be the most suitable, reporting an average MAP isolation rate of 3.99% (886/22,154) and an average contamination rate of 0.17% (38/22,154). This systematic review highlights the need for further refinement of decontamination protocols to minimize the loss of viable MAP during processing of bovine fecal and environmental samples.
{"title":"Decontamination protocols on bovine fecal and environmental samples for Mycobacterium avium ssp. paratuberculosis growth in solid media","authors":"Nathalia M Correa Valencia, José Miguel Hernández Agudelo, Jorge A Fernández Silva","doi":"10.4206/ajvs.561.01","DOIUrl":"https://doi.org/10.4206/ajvs.561.01","url":null,"abstract":"Bacteriological culture of Mycobacterium avium subsp. paratuberculosis (MAP) is considered the gold standard to confirm its presence in several matrices for Johne’s disease diagnosis. Whether it is a liquid or solid culture, a problem with MAP culture is that non-interpretable results arise because of overgrowth by other microorganisms, making MAP growth and identification more difficult or impossible. We systematically reviewed published decontamination protocols and their effects on MAP culture from bovine fecal and environmental samples on solid media. Based on our findings, we suggest a step-by-step decontamination protocol. The OVID®/MEDLINE, PubMed®, SciELO Citation Index®, and Redalyc® platforms as well as the International Colloquium on Paratuberculosis (ICP) proceedings and the reference lists were reviewed to identify relevant studies. The inclusion criteria considered articles published in English, Portuguese, French, German, Spanish, and peer-reviewed journals. The exclusion criteria included unrelated topics, species other than bovines, other than environmental/faecal samples, other than diagnostic techniques of interest, and non-original articles. Definitive studies were obtained through the authors’ consensus regarding their eligibility and quality. In total, 1,004 publications matched the search terms, and 27 articles met the inclusion criteria, of which 45 derived and reported 15 different decontamination protocols. The centrifugation-one-step hexadecylpyridinium chloride protocol, which used over 22,154 fecal samples in three studies, was found to be the most suitable, reporting an average MAP isolation rate of 3.99% (886/22,154) and an average contamination rate of 0.17% (38/22,154). This systematic review highlights the need for further refinement of decontamination protocols to minimize the loss of viable MAP during processing of bovine fecal and environmental samples.","PeriodicalId":56042,"journal":{"name":"Austral Journal of Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139535064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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