Circulating Antigen of Toxoplasma gondii in Patients with AIDS: Significance of Detection and Structural Properties

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI:10.1016/S0176-6724(88)80167-6

Andreas Hassl , Horst Aspöck , Heinz Flamm

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引用次数: 16

Abstract

232 sera and 40 cerebrospinal fluid samples of altogether 125 patients in stages III or IV of a HIV-infection were tested for circulating antigen of Toxoplasma gondii by means of a three-layer enzyme-linked immunosorbent assay. Circulating antigen was detected in 32 sera of 20 patients (= 16% of all persons investigated). These ELISA results were reexamined by an Immunoblot following a SDS-PAGE and confirmed in most cases. In addition, this test system led to a partial characterization of the circulating antigen; it consists of at least two proteins with atomic mass units of 27 and 57 kd respectively.

The antigenemia was correlated with IgG- and IgM-antibody titres, with clinical symptoms, and with pathological findings also. Our results indicate that the detection of circulating antigen in sera offers a rapid and efficient method for the diagnosis of an acute toxoplasmosis in AIDS-patients.

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艾滋病患者刚地弓形虫循环抗原的检测及其结构特性的意义

采用三层酶联免疫吸附法对125例hivⅲ期或ⅳ期患者的232份血清和40份脑脊液进行了刚地弓形虫循环抗原检测。在20例患者的32份血清中检测到循环抗原(占所有调查人员的16%)。这些ELISA结果在SDS-PAGE后通过免疫印迹重新检查，并在大多数病例中得到证实。此外，该测试系统导致循环抗原的部分表征;它由至少两个原子质量分别为27和57 kd的蛋白质组成。抗原血症与IgG抗体和igm抗体滴度、临床症状和病理结果相关。结果表明，血清循环抗原检测为艾滋病患者急性弓形虫病的诊断提供了一种快速有效的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology

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