Metabolomic and phenotypic effects of ocean acidification on cuttlefish differ across early life stages

IF 3.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Marine environmental research Pub Date : 2025-03-01 Epub Date: 2025-02-19 DOI:10.1016/j.marenvres.2025.107013

Antoine Minet , Steven Melvin , Marc Metian , Angus Taylor , François Oberhänsli , Christel Lefrançois , Peter Swarzenski , Paco Bustamante , Thomas Lacoue-Labarthe

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Abstract

Ocean acidification (OA) affects the physiology and behaviour of some marine organisms, impacting their development and metabolism during vulnerable early-life stages. Among them, the embryo of the cuttlefish develops for about two months in encapsulated eggs with harsh perivitelline conditions of hypoxia and hypercapnia, potentially worsened by OA. In this study, common cuttlefish Sepia officinalis embryos and juveniles, were exposed to five pH conditions (pH_T 8.08 to 7.43). Growth, development and metabolite profiles were explored during the embryonic development period up to 10 days-post-hatching. Our results show delayed embryonic development and decreased hatching success at pH 7.43, but no effect on juvenile weight upon hatching. The ¹H Nuclear Magnetic Resonance (NMR) spectroscopy revealed that decreasing pH affected metabolites profiles in embryos until a metabolic suppression was observed at pH 7.43. The O₂ consumption in 10d-old juveniles did not change with pH whereas metabolites indicated a switch to anaerobic metabolism under low pH. Overall, our results suggest that the transition from the encapsulated embryonic stage to the free juvenile life shapes a metabolomic reprogramming more drastically than ocean acidification.

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海洋酸化对墨鱼的代谢组学和表型影响在生命早期阶段有所不同

海洋酸化（OA）影响一些海洋生物的生理和行为，影响它们在脆弱的生命早期阶段的发育和代谢。其中，墨鱼的胚胎在被封装的卵中发育约两个月，卵周缺氧和高碳酸血症的恶劣条件，可能因OA而恶化。本研究将普通墨鱼（Sepia officinalis）的胚胎和幼鱼分别置于pH值为8.08 ~ 7.43的5种pH条件下。在孵化后10天的胚胎发育期间，研究了生长、发育和代谢物特征。结果表明，pH值为7.43时，胚胎发育延迟，孵化成功率降低，但对孵化时幼鱼体重没有影响。1H核磁共振（NMR）波谱显示，pH值降低会影响胚胎代谢物谱，直到pH值为7.43时才出现代谢抑制。10d龄幼鱼的氧气消耗不随pH值变化，而代谢物则表明在低pH值条件下，幼鱼会向厌氧代谢转变。总的来说，我们的研究结果表明，从被封装的胚胎阶段到自由的幼鱼生命的转变形成了比海洋酸化更剧烈的代谢组重编程。

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来源期刊

Marine environmental research 环境科学-毒理学

CiteScore

5.90

自引率

3.00%

发文量

217

审稿时长

46 days

期刊介绍： Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes. Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following: – The extent, persistence, and consequences of change and the recovery from such change in natural marine systems – The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems – The biogeochemistry of naturally occurring and anthropogenic substances – Models that describe and predict the above processes – Monitoring studies, to the extent that their results provide new information on functional processes – Methodological papers describing improved quantitative techniques for the marine sciences.