Transposon mutagenesis identifies acid resistance and biofilm genes as Shigella sonnei virulence factors in Caenorhabditis elegans infection

IF 2.2 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical and biophysical research communications Pub Date : 2025-03-01 DOI:10.1016/j.bbrc.2025.151546

Bao Chi Wong , Fong Yoke Ling , Qasim Ayub , Hock Siew Tan

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Abstract

Identifying essential genes in bacterial pathogens during infection can enhance knowledge and provide novel targets for antimicrobial agents’ development. Currently, only Shigella flexneri essential genes during in vitro growth have been experimentally identified. This study used transposon insertion sequencing (TIS) to identify Shigella sonnei essential genes during Caenorhabditis elegans infection. 498 genes were predicted to be essential in S. sonnei during growth on nutrient-rich media. Some genes previously predicted to be essential in Shigella were found non-essential in S. sonnei, such as acetyl metabolism genes (aceEF, lpdA) and sulphate transport genes (cysA, cyst, cysW). Finally, 217 genes were predicted as S. sonnei virulence genes during infection, including acid resistance and biofilm formation genes which was not linked to S. sonnei virulence previously.

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转座子诱变鉴定耐酸基因和生物膜基因是秀丽隐杆线虫感染的索内志贺氏菌毒力因子

在感染过程中鉴定细菌病原体的必需基因可以增加知识并为抗菌药物的开发提供新的靶点。目前，只有福氏志贺氏菌在体外生长过程中的必需基因被实验鉴定出来。本研究采用转座子插入测序（TIS）技术鉴定秀丽隐杆线虫感染过程中索内志贺氏菌必需基因。预计有498个基因对sonnei在富营养培养基上的生长至关重要。一些先前预测为志贺氏菌必需的基因在sonnei中被发现不是必需的，如乙酰代谢基因（aceEF, lpdA）和硫酸盐转运基因（cysA，囊肿，cysW）。最终，217个基因在感染过程中被预测为sonnei链球菌毒力基因，其中包括先前未与sonnei链球菌毒力相关的抗酸基因和生物膜形成基因。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics