Unveiling novel oxygen-dependent docosahexaenoic acid biosynthesis pathway in Crypthecodinium cohnii via dual-function △4-fatty acid desaturases

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2025-02-25 DOI:10.1016/j.biortech.2025.132292

Yi-Tong Yao , Xiao Zhang , Chen-Yu Wang , Yu-He Zhang , Da-Wei Li, Wei-Dong Yang, Hong-Ye Li, Li-Gong Zou

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Abstract

Crypthecodinium cohnii, a protist renowned for its high docosahexaenoic acid (DHA) production, has an unclear mechanism for converting docosapentaenoic acid (DPA) into DHA. This study employed transcriptomic analysis to investigate the effect of excessive oxygen limitation (EOL) on DHA biosynthesis, uncovering a novel oxygen-dependent pathway. The use of intermittent oxygen limitation (IOL) strategy significantly boosted DHA production. Five △4-fatty acid desaturase (FAD4) genes were identified, with CcFAD4_52534 exhibiting the highest catalytic efficiency and dual-functionality, converting eicosapentaenoic acid (EPA) to DPA, and subsequently to DHA. This study integrates transcriptomic insights, the discovery of the bifunctional CcFAD4_52534 enzyme, and the optimized IOL strategy, offering transformative potential for sustainable and high-yield DHA production through tailored genetic engineering in C. cohnii. This approach bridges ecological understanding with industrial innovation.

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隐翅虫（Crypthecodinium cohnii）是一种以生产大量二十二碳六烯酸（DHA）而闻名的原生动物，但其将二十二碳五烯酸（DPA）转化为 DHA 的机制尚不清楚。本研究利用转录组分析来研究过度氧限制（EOL）对 DHA 生物合成的影响，发现了一种新的氧气依赖途径。使用间歇性氧限制（IOL）策略显著提高了DHA的产量。研究发现了五个△4-脂肪酸去饱和酶（FAD4）基因，其中CcFAD4_52534具有最高的催化效率和双重功能，可将二十碳五烯酸（EPA）转化为DPA，进而转化为DHA。这项研究综合了转录组学的见解、双功能 CcFAD4_52534 酶的发现以及优化的 IOL 策略，通过对 C. cohnii 进行量身定制的基因工程，为可持续高产 DHA 生产提供了变革性的潜力。这种方法是生态理解与工业创新的桥梁。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.