Małgorzata Bulanda , Zenon Weęgrzynowicz , Marek Ciurak , Janusz Kowalczyk , Gotfryd Kupryszewski , Maria Gruszka , Piotr B. Heczko , Gerhard Pulverer
{"title":"A New Chromogenic Assay for Direct Detection of Staphylocoagulase","authors":"Małgorzata Bulanda , Zenon Weęgrzynowicz , Marek Ciurak , Janusz Kowalczyk , Gotfryd Kupryszewski , Maria Gruszka , Piotr B. Heczko , Gerhard Pulverer","doi":"10.1016/S0176-6724(88)80147-0","DOIUrl":null,"url":null,"abstract":"<div><p>Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of <em>Staphylococcus aureus</em>. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.</p></div><div><p>Trotz mancher Nachteile wird der Röhrchentest generell zum Nachweis der Staphylokokken-Koagulase in der medizinischen Mikrobiologie verwendet, um die Spezies <em>Staphylococcus aureus</em> zu identifizieren. In vorliegender Arbeit wird eine neue chromogene Methode zum Nachweis der Koagulase direkt in der Staphylokokken-Kultur vorgestellt und mit dem Standard-Röhrchentest verglichen. Der chromogene Nachweistest erwies sich dem Standard-Röhrchentest als ebenbürtig, kann aber in wenigen Stunden ohne apparative Hilfe abgelesen werden.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 115-121"},"PeriodicalIF":0.0000,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80147-0","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176672488801470","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of Staphylococcus aureus. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.
Trotz mancher Nachteile wird der Röhrchentest generell zum Nachweis der Staphylokokken-Koagulase in der medizinischen Mikrobiologie verwendet, um die Spezies Staphylococcus aureus zu identifizieren. In vorliegender Arbeit wird eine neue chromogene Methode zum Nachweis der Koagulase direkt in der Staphylokokken-Kultur vorgestellt und mit dem Standard-Röhrchentest verglichen. Der chromogene Nachweistest erwies sich dem Standard-Röhrchentest als ebenbürtig, kann aber in wenigen Stunden ohne apparative Hilfe abgelesen werden.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
