Could Exotic Birds Play a Significant Role in the Emergence of Antibiotic-Resistant Microorganisms?

Abstract

Despite limited studies, free-living birds are considered reservoirs of several diseases, including zoonotic diseases. Thus, we aimed to investigate the presence and characteristics of Campylobacter spp., Escherichia coli, and Salmonella spp. in wild birds assessing their potential risks to human and animal health. Fecal samples were collected from wild and exotic rescued birds, as well as exotic parrots from commercial breeding facilities. Campylobacter spp. and Salmonella spp. were not detected in the samples. E. coli was biochemically confirmed in 58.33% of the samples, resulting nine distinct isolates. PCR analysis identified no enteroinvasive or enteropathogenic E. coli strains, but two isolates were characterized as avian pathogenic E. coli (APEC). The isolates were inoculated into chicken embryos to determine their pathogenicity index, utilizing commensal and pathogenic E. coli strains as controls. All isolates demonstrated higher pathogenicity compared to probiotic bacteria and commensal E. coli. Specifically, two isolates exhibited pathogenicity levels comparable to the positive control (APEC ST131). Furthermore, for the minimum inhibitory concentration (MIC) test, we evaluated 7 antibiotics: ampicillin, ceftazidime, cefotaxime, ciprofloxacin, levofloxacin, colistin, and sulfamethoxazole combined with trimethoprim. Four isolates were classified as multidrug-resistant and the highest level of resistance was to fluoroquinolones. This work demonstrated that wild or captive exotic birds can excrete potentially pathogenic and/or multidrug-resistant E. coli, which may impact the health of other animals and humans.