Characterization of Campylobacter fetus adherence, invasiveness, and ultrastructural damage on bovine oviductal cells

IF 3.5 3区医学 Q3 IMMUNOLOGY Microbial pathogenesis Pub Date : 2025-05-01 Epub Date: 2025-02-26 DOI:10.1016/j.micpath.2025.107429

Claudia Inés Cagnoli , María Laura Chiapparrone , Francisco Acuña , Claudio Santiago Cacciato , Marcelo Gastón Rodríguez , Juan Florencio Aller , María del Carmen Catena

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Abstract

Bovine campylobacteriosis is a distributed worldwide disease caused by Campylobacter fetus. It is a sexual transmitted disease that affects reproductive health in cattle. The objective of this study was to use bovine oviduct primary cell cultures as a pathogenicity model to study the virulence of Campylobacter fetus fetus and Campylobacter fetus venerealis. Both subspecies showed the ability to adhere to, invade and cause cytopathogenic effect in oviductal cells. Transmission electron microscopy revealed significant ultrastructural damage, including cytoplasmic vacuolization, nuclear condensation and mitochondrial alterations. These mitochondrial changes, such enlargement and fragmentation, suggest potential impacts on cell viability and host cellular metabolism. This study is the first to investigate the pathogenicity of both subspecies in primary bovine oviductal epithelial cells. These findings demonstrate the virulence mechanism of C. fetus in the reproductive tract, providing insights into oviductal pathogenicity and potential impacts on bovine fertility.

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弯曲杆菌胎儿粘附性、侵袭性和牛输卵管细胞超微结构损伤的表征。

牛弯曲杆菌病是由胎儿弯曲杆菌引起的一种分布于世界各地的疾病。它是一种影响牛生殖健康的性传播疾病。本研究的目的是利用牛输卵管原代细胞作为致病性模型，研究胎儿弯曲杆菌和胎儿性病弯曲杆菌的毒力。这两个亚种都能粘附、侵入输卵管细胞并引起细胞致病作用。透射电子显微镜显示出明显的超微结构损伤，包括细胞质空泡化、核浓缩和线粒体改变。这些线粒体变化，如增大和碎裂，表明可能对细胞活力和宿主细胞代谢产生影响。本研究首次调查了这两个亚种在原代牛输卵管上皮细胞中的致病性。这些发现证明了胎生球虫在生殖道中的致病机制，为了解输卵管致病性和对牛生育力的潜在影响提供了思路。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)