Cytotype and local adaptation drive phenotypic variation in two subspecies of big sagebrush (Artemisia tridentata)

Abstract

Big sagebrush (Artemisia tridentata) is a widespread and locally dominant shrub throughout many ecosystems in western North America. A. tridentata ssps. tridentata and wyomingensis are two subspecies whose populations occupy the warm-arid regions of the species range and whose trailing edge is threatened by climate change. Previous studies have presented conflicting results in relation to the genetic control of physiological variation in A. tridentata. Understanding how different genetic factors contribute to physiological variation can provide insight into how these two subspecies may respond to future climate change. To explore possible variation among and within two subspecies of A. tridentata, we measured physiological and morphological traits in A. t. tridentata and A. t. wyomingensis during mid-summer (July), seven years after establishment in a common garden. Contributions to trait variation were quantified for both genetic (subspecies and cytotype) and environmental (climate-of-origin) factors. Measurements revealed an unequal contribution to phenotypic variation by subspecies, cytotype, and climate-of-origin. Ploidy and climate-of-origin were more important than subspecies in driving phenotypic variation in A. tridentata. These findings suggest that A. tridentata has a highly plastic drought response, or that culling (mortality over time due to environmental factors) in the common garden over seven years has led to a lack of genetic diversity within the garden. Understanding what factors drive phenotypic expression in big sagebrush can provide better insight into how climate change may affect migration and extirpation and may aid in the effectiveness of restoration efforts.