Simple Method for Efficient RNA Extraction From Arabidopsis Embryos.

Abstract

Plant embryos are contained within seeds. Isolating them is crucial when endosperm and seed coat tissues interfere with the study of mutant genetic functions due to differing genotypes between maternal and embryonic tissues. RNA extraction from plant embryonic tissue presents particular challenges due to the high activity of RNases, the composition of the seed, and the risk of RNA degradation. The developmental stage of the embryo is a key aspect of successful isolation and RNA extraction due to the size and amount of tissue. Proper handling during RNA extraction is critical to maintain RNA integrity and prevent degradation. While commercial kits offer various methods for RNA extraction from embryos, homemade protocols provide valuable advantages, including cost-effectiveness and accessibility for labs with limited funding. Here, we present a simple and efficient protocol for extracting RNA from isolated Arabidopsis thaliana embryos at the torpedo/cotyledon stage using a homemade extraction buffer previously reported for styles of Nicotiana alata. Key features • This straightforward homemade protocol builds upon established methods for embryo isolation [1] and RNA extraction [2,3]. • It enables the extraction of high-quality RNA from Arabidopsis embryos at the torpedo stage. • The protocol is designed to be both simple and cost-effective, making it an excellent option for labs with limited funding. Graphical overview Simple method for RNA extraction from isolated Arabidopsis embryos using a homemade extraction buffer.