Insights into in-situ free nitrous acid induced extracellular polymeric substances changes and membrane fouling mitigation in a nitritation membrane bioreactor

IF 12.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Water Research Pub Date : 2025-03-05 DOI:10.1016/j.watres.2025.123446

Yishuai Jiang , Xiaoyuan Zhang , Hiroshige Shiota , Wun Jern Ng

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Abstract

This study investigated the effectiveness of free nitrous acid (FNA) on mitigating membrane fouling, with the associated mechanisms, in two nitritation membrane bioreactors (MBRs) operated with Nitrosomonas-enriched culture. Results showed that FNA stress, regulated by pH and nitrite concentration, maintained a low-level fouling as opposed to the control MBR where trans-membrane pressure (TMP) exceeded 30 kPa. Compared to the control MBR, production of biofilm in the FNA stressed MBR was reduced by 68.1% in terms of mass and 78.2% in terms of thickness. Suspended biomass and biofilm extracellular polymeric substances (EPS) characterized by liquid chromatography (LC-OCD-OND) indicated FNA stress reduced the amount of low molecular weight neutrals and hydrophobic dissolved organic carbon. These components would have had high fouling potential. Excitation emission matrix (EEM) fluorescence contours indicated that exposure to FNA stimulated the production of tyrosine-like proteins but reduced those of SMP like and humic acid-like substances. This could have affected the adhesion between bacteria and membrane and so contributed to the reduced biofilm and fouling. X-ray photoelectron spectroscopy (XPS) analysis revealed marked differences in intensities of the main functionalities in the EPS for both sludge and biofilm, due to the oxidative effect of FNA, e.g. FNA stress resulted in more aliphatic C-OH, amines and amides while the control had more C=O, amino acids and amino sugars. This study showed that in-situ generated FNA could be employed to mitigate membrane fouling effectively via its biocidal and oxidative effect.

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硝化膜生物反应器中游离亚硝酸盐诱导的胞外聚合物质变化和膜污染缓解的研究进展

本文研究了游离亚硝酸盐（FNA）在两个亚硝化膜生物反应器（mbr）中对膜污染的缓解效果及其相关机制。结果表明，与跨膜压力（TMP）超过30 kPa的对照MBR相比，受pH和亚硝酸盐浓度调节的FNA胁迫维持了较低的污染水平。与对照MBR相比，FNA胁迫下MBR的生物膜产量在质量上减少了68.1%，在厚度上减少了78.2%。液相色谱（LC-OCD-OND）表征的悬浮物生物量和生物膜胞外聚合物（EPS）表明，FNA胁迫降低了低分子量中性物质和疏水溶解有机碳的数量。这些组件有很高的污染潜力。激发发射基质（EEM）荧光曲线显示，FNA刺激了酪氨酸样蛋白的产生，但减少了SMP样和腐植酸样物质的产生。这可能会影响细菌与膜之间的粘附，从而导致生物膜的减少和污染。x射线光电子能谱（XPS）分析显示，由于FNA的氧化作用，污泥和生物膜的EPS中主要功能的强度存在显著差异，例如FNA胁迫导致更多的脂肪族C- oh、胺和酰胺，而对照组则有更多的C=O、氨基酸和氨基糖。本研究表明，原位生成的FNA可以通过其生物杀灭和氧化作用有效地缓解膜污染。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.