Resilient STR loci under gamma radiation: A preliminary study on DNA stability in buccal swabs

IF 1.8 3区工程技术 Q3 CHEMISTRY, INORGANIC & NUCLEAR Applied Radiation and Isotopes Pub Date : 2025-06-01 Epub Date: 2025-03-06 DOI:10.1016/j.apradiso.2025.111762

Amani Kraishan , Ahmad M. Refaat , Jenan A. Almhaini , Alaa Saeed Bazuhair , Saeed M. Al-Qahtani , Marwan A. Althomali , Hatem Al-Ameryeen , Ali H. Alomari , Abdul-Wali Ajlouni

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Abstract

This study explores how gamma radiation affects DNA concentration and the stability of autosomal short tandem repeat (STR) markers in buccal swab samples. A total of 160 swabs from 20 volunteers were exposed to therapeutic radiation doses ranging from 1 to 80 Gy. The results revealed a clear dose-dependent decrease in DNA concentration, indicating significant DNA damage as radiation increased. Despite this, the autosomal STR markers remained stable across all doses, demonstrating their robustness against radiation-induced damage. These findings are crucial for clinical and forensic applications, confirming that STR profiling remains reliable for cancer patients undergoing radiation therapy and in forensic cases involving irradiated DNA. The study emphasizes the value of STR markers in genetic analysis under challenging conditions and highlights the need for further research on their long-term stability in diverse biological contexts.

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伽马辐射下弹性STR基因座：口腔拭子DNA稳定性的初步研究

本研究探讨了伽马辐射如何影响口腔拭子样本中DNA浓度和常染色体短串联重复序列（STR）标记的稳定性。来自20名志愿者的160份拭子接受了1至80戈瑞的治疗性辐射剂量。结果显示，DNA浓度明显呈剂量依赖性下降，表明随着辐射增加，DNA损伤显著。尽管如此，常染色体STR标记在所有剂量下都保持稳定，表明它们对辐射引起的损伤具有稳健性。这些发现对于临床和法医应用至关重要，证实STR分析对于接受放射治疗的癌症患者和涉及辐照DNA的法医案件仍然是可靠的。该研究强调了STR标记在具有挑战性条件下的遗传分析中的价值，并强调需要进一步研究其在不同生物环境下的长期稳定性。

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来源期刊

Applied Radiation and Isotopes 工程技术-核科学技术

CiteScore

3.00

自引率

12.50%

发文量

406

审稿时长

13.5 months

期刊介绍： Applied Radiation and Isotopes provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and peaceful application of nuclear, radiation and radionuclide techniques in chemistry, physics, biochemistry, biology, medicine, security, engineering and in the earth, planetary and environmental sciences, all including dosimetry. Nuclear techniques are defined in the broadest sense and both experimental and theoretical papers are welcome. They include the development and use of α- and β-particles, X-rays and γ-rays, neutrons and other nuclear particles and radiations from all sources, including radionuclides, synchrotron sources, cyclotrons and reactors and from the natural environment. The journal aims to publish papers with significance to an international audience, containing substantial novelty and scientific impact. The Editors reserve the rights to reject, with or without external review, papers that do not meet these criteria. Papers dealing with radiation processing, i.e., where radiation is used to bring about a biological, chemical or physical change in a material, should be directed to our sister journal Radiation Physics and Chemistry.