Overexpression of the Apoe gene in the frontal cortex of mice causes sex-dependent changes in learning, attention, and anxiety-like behavior.

IF 1.8 4区 医学 Q4 NEUROSCIENCES Learning & memory Pub Date : 2025-03-07 Print Date: 2025-03-01 DOI:10.1101/lm.054064.124
Lizbeth Ramos, Abigail E Harr, Finian L Zakas, Samuel R Essig, Griffen J Kempskie, Nelly A Fadil, Makayla G Schmid, Madison D Pompy, Michael C Curley, Lisa A Gabel, Henry L Hallock
{"title":"Overexpression of the <i>Apoe</i> gene in the frontal cortex of mice causes sex-dependent changes in learning, attention, and anxiety-like behavior.","authors":"Lizbeth Ramos, Abigail E Harr, Finian L Zakas, Samuel R Essig, Griffen J Kempskie, Nelly A Fadil, Makayla G Schmid, Madison D Pompy, Michael C Curley, Lisa A Gabel, Henry L Hallock","doi":"10.1101/lm.054064.124","DOIUrl":null,"url":null,"abstract":"<p><p>Apolipoprotein E (ApoE) is a protein that is important for lipid storage, transport, and metabolism. <i>APOE</i> gene variants are associated with Alzheimer's disease, as well as attentional function in healthy humans. Previous research has shown that <i>Apoe</i> transcription is increased following stimulation of the pathway between the locus coeruleus (LC) and frontal cortex (FC) in mice. This result suggests that <i>Apoe</i> may affect attentional function by virtue of its expression in circuits that control attention. Does <i>Apoe</i> causally regulate attention, or is its expression simply a byproduct of neuronal activity in the LC and FC? To answer this question, we synthetically induced <i>Apoe</i> transcription in the FC of male and female mice, and subsequently tested their ability to learn a touchscreen-based rodent version of the continuous performance test of sustained attention (the rCPT). We found that increased <i>Apoe</i> transcription impaired performance when attentional demand was increased in male mice, while in female mice, increased <i>Apoe</i> transcription significantly accelerated rCPT learning. We further found that this increase in <i>Apoe</i> transcription affected one metric of the open field test, as well as cellular activity in the FC in a sex-dependent manner. The results of this study provide insight into how <i>Apoe</i> causally regulates translationally relevant behaviors in rodent models.</p>","PeriodicalId":18003,"journal":{"name":"Learning & memory","volume":"32 3","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Learning & memory","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1101/lm.054064.124","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"Print","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Apolipoprotein E (ApoE) is a protein that is important for lipid storage, transport, and metabolism. APOE gene variants are associated with Alzheimer's disease, as well as attentional function in healthy humans. Previous research has shown that Apoe transcription is increased following stimulation of the pathway between the locus coeruleus (LC) and frontal cortex (FC) in mice. This result suggests that Apoe may affect attentional function by virtue of its expression in circuits that control attention. Does Apoe causally regulate attention, or is its expression simply a byproduct of neuronal activity in the LC and FC? To answer this question, we synthetically induced Apoe transcription in the FC of male and female mice, and subsequently tested their ability to learn a touchscreen-based rodent version of the continuous performance test of sustained attention (the rCPT). We found that increased Apoe transcription impaired performance when attentional demand was increased in male mice, while in female mice, increased Apoe transcription significantly accelerated rCPT learning. We further found that this increase in Apoe transcription affected one metric of the open field test, as well as cellular activity in the FC in a sex-dependent manner. The results of this study provide insight into how Apoe causally regulates translationally relevant behaviors in rodent models.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
求助全文
约1分钟内获得全文 去求助
来源期刊
Learning & memory
Learning & memory 医学-神经科学
CiteScore
3.60
自引率
5.00%
发文量
45
审稿时长
6-12 weeks
期刊介绍: The neurobiology of learning and memory is entering a new interdisciplinary era. Advances in neuropsychology have identified regions of brain tissue that are critical for certain types of function. Electrophysiological techniques have revealed behavioral correlates of neuronal activity. Studies of synaptic plasticity suggest that some mechanisms of memory formation may resemble those of neural development. And molecular approaches have identified genes with patterns of expression that influence behavior. It is clear that future progress depends on interdisciplinary investigations. The current literature of learning and memory is large but fragmented. Until now, there has been no single journal devoted to this area of study and no dominant journal that demands attention by serious workers in the area, regardless of specialty. Learning & Memory provides a forum for these investigations in the form of research papers and review articles.
期刊最新文献
Behavioral microanalyses refine sign-tracking characterization and uncover different response dynamics during omission and extinction learning. Overexpression of the Apoe gene in the frontal cortex of mice causes sex-dependent changes in learning, attention, and anxiety-like behavior. Large-scale study of human memory for meaningful narratives. No effect of partial reinforcement on fear extinction learning and memory in C57BL/6J mice. Goal orientation shifts attentional focus and impairs reward-motivated memory.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1