Optimisation and utilization of deep eutectic solvents for the green extraction of anticancer alkaloids from Catharanthus roseus.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-03-08 DOI:10.1080/14786419.2025.2476199

Avjot Kaur, Ramandeep Kaur, Urvashi Bhardwaj, Parul Sharma

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引用次数: 0

Abstract

The extraction of vinca alkaloids, such as vinblastine and vincristine, has traditionally been a challenge due to their low concentrations in natural sources and lengthy, low-yielding procedures. In this study, we explored a high-yield, efficient method for extracting vinblastine and vincristine from Catharanthus roseus using deep eutectic solvents (DES). We prepared various choline chloride (ChCl) based DESs with different hydrogen bond donors (HBD) and used them to extract leaves and flowers of C. roseus under ultrasonication. The extracts were evaluated for vinblastine and vincristine content using UV-spectroscopic analysis. Using response surface methodology, various parameters were optimised, including water content, sonication time, and solid-to-liquid ratio. Under optimised conditions, DES based on organic acids was found to extract 27.94 μg/ml of vinblastine in Choline chloride: citric acid DES and 30.84 μg/ml of vincristine in Choline chloride: oxalic acid. These results highlight the eco-friendliness of Choline chloride: citric acid and Choline chloride: oxalic acid DESs, which are effective, sustainable alternatives to conventional solvents for extracting these alkaloids from C. roseus, contributing to our collective environmental consciousness.

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.