Optimisation and utilization of deep eutectic solvents for the green extraction of anticancer alkaloids from Catharanthus roseus.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-03-08 DOI:10.1080/14786419.2025.2476199

Avjot Kaur, Ramandeep Kaur, Urvashi Bhardwaj, Parul Sharma

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Abstract

The extraction of vinca alkaloids, such as vinblastine and vincristine, has traditionally been a challenge due to their low concentrations in natural sources and lengthy, low-yielding procedures. In this study, we explored a high-yield, efficient method for extracting vinblastine and vincristine from Catharanthus roseus using deep eutectic solvents (DES). We prepared various choline chloride (ChCl) based DESs with different hydrogen bond donors (HBD) and used them to extract leaves and flowers of C. roseus under ultrasonication. The extracts were evaluated for vinblastine and vincristine content using UV-spectroscopic analysis. Using response surface methodology, various parameters were optimised, including water content, sonication time, and solid-to-liquid ratio. Under optimised conditions, DES based on organic acids was found to extract 27.94 μg/ml of vinblastine in Choline chloride: citric acid DES and 30.84 μg/ml of vincristine in Choline chloride: oxalic acid. These results highlight the eco-friendliness of Choline chloride: citric acid and Choline chloride: oxalic acid DESs, which are effective, sustainable alternatives to conventional solvents for extracting these alkaloids from C. roseus, contributing to our collective environmental consciousness.

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绿色提取长春花抗癌生物碱的深共晶溶剂优化与利用。

长春花生物碱的提取，如长春花碱和长春新碱，传统上是一个挑战，因为它们在天然来源中的浓度低，时间长，产量低。本研究探索了一种高效、高效的深共晶溶剂萃取长春花碱和长春新碱的方法。采用不同的氢键供体（HBD）制备了不同的氯化胆碱（ChCl）基脱脂剂（DESs），并将其应用于超声提取玫瑰香叶和花。紫外分光光度法测定长春新碱和长春新碱的含量。利用响应面法，优化了各种参数，包括含水量、超声时间和固液比。在优化条件下，基于有机酸的DES从氯化胆碱：柠檬酸中提取长春新碱27.94 μg/ml，从氯化胆碱：草酸中提取长春新碱30.84 μg/ml。这些结果强调了氯化胆碱：柠檬酸和氯化胆碱：草酸DESs的生态友好性，是传统溶剂提取玫瑰中这些生物碱的有效、可持续的替代品，有助于我们的集体环保意识。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.