A Comparison of the Structural Changes and IgG Immunobinding Activity of Parvalbumin in Salangid Icefish (Neosalanx taihuensis) After Glycation and Ultra-High Pressure Treatment.

IF 5.1 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Foods Pub Date : 2025-03-02 DOI:10.3390/foods14050856
Ying Huang, Yang Hu, Jiawei Liu, Haiying Liu
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Abstract

The aim of this study was to compare the effects of glycation and ultra-high pressure (UHP) treatment on the structure and IgG immunobinding activity of Salangidae icefish PV. The Circular Dichroism (CD) and Fluorescence Spectroscopy (FS) findings indicated that the glycation significantly affected both the secondary and tertiary structures of PV. However, the impact of UHP processing on the structure of PV was found to be less significant compared to the glycation. Western Blot analysis also revealed that the glycation markedly reduced the antigen specificity of PV. Conversely, UHP treatments at 300 MPa and 400 MPa slightly decreased the antigen specificity, whereas lower or excessively high pressures did not have a substantial impact. This research contributes valuable insights into strategies for reducing the allergenic potential of Salangid icefish.

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糖基化与超高压处理对太湖沙鳗小白蛋白结构变化及IgG免疫结合活性的影响
本研究旨在比较糖基化和超高压(UHP)处理对Salangidae冰鱼PV的结构和IgG免疫结合活性的影响。圆二色性(CD)和荧光光谱(FS)结果表明糖基化对PV的二级和三级结构都有显著影响。然而,与糖基化相比,UHP处理对PV结构的影响并不显著。Western Blot分析也显示糖基化显著降低了PV的抗原特异性。相反,300 MPa和400 MPa的超高压处理会略微降低抗原特异性,而较低或过高的压力则不会产生实质性影响。本研究为降低Salangid冰鱼致敏潜力的策略提供了有价值的见解。
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来源期刊
Foods
Foods Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
15.40%
发文量
3516
审稿时长
15.83 days
期刊介绍: Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Ÿ manuscripts regarding research proposals and research ideas will be particularly welcomed Ÿ electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material Ÿ we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds
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