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Enhancement of Mechanical Properties of Zein-Based Nanofibers by Incorporation of Millet Gliadin 通过加入小米胶蛋白提高玉米蛋白纳米纤维的机械性能
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182900
Shumin Wang, Pengjie Wang, Siyuan Liu, Ran Wang, Yixuan Li, Xiaoyu Wang, Fazheng Ren, Jie Luo, Bing Fang
In this work, a novel reinforcing filler, millet gliadin (MG), was used for the improvement of the mechanical properties of zein nanofibers. The structural and physicochemical properties of MG were compared with those of zein, and the influence of MG on the morphology, physical properties, and molecular structure of zein nanofibers was investigated. The results indicated that MG has an obviously smaller weight-average molecular weight (7623) in comparison to zein (13,330). Transmission electron microscopy showed that zein molecules more easily form aggregates with larger diameters than MG molecules in acetic acid. At a concentration of 30% (w/v), MG exhibited a significantly higher viscosity (0.66 ± 0.03 Pa·s) than zein (0.32 ± 0.01 Pa·s), indicating the stronger interactions of MG molecules. With the incorporation of MG, the tensile strength was significantly increased to 49.32 MPa (ZM-1/2), which is 2.08 times and 4.45 times higher than that of pure zein nanofibers (ZM-1/0) and MG nanofibers (ZM-0/1-1), respectively. Moreover, zein/MG composite nanofibers exhibited improved water stability. Fourier transform infrared spectra showed evidence of the hydrogen bonding interaction between zein and MG. Therefore, MG is a good candidate for use as a natural reinforcing filler in electrospun nanofibers made of biopolymers.
本研究采用一种新型增强填料--小米胶蛋白(MG)来改善玉米蛋白纳米纤维的机械性能。比较了 MG 与玉米蛋白的结构和理化性质,并研究了 MG 对玉米蛋白纳米纤维的形态、物理性质和分子结构的影响。结果表明,MG 的平均分子量(7623)明显小于玉米蛋白(13330)。透射电子显微镜显示,在醋酸中,玉米蛋白分子比 MG 分子更容易形成直径更大的聚集体。在浓度为 30% (w/v) 时,MG 的粘度(0.66 ± 0.03 Pa-s)明显高于玉米蛋白(0.32 ± 0.01 Pa-s),这表明 MG 分子的相互作用更强。加入 MG 后,拉伸强度显著提高,达到 49.32 MPa(ZM-1/2),分别是纯玉米蛋白纳米纤维(ZM-1/0)和 MG 纳米纤维(ZM-0/1-1)的 2.08 倍和 4.45 倍。此外,玉米蛋白/MG 复合纳米纤维还具有更好的水稳定性。傅立叶变换红外光谱显示,玉米蛋白和 MG 之间存在氢键相互作用。因此,MG 是生物聚合物电纺纳米纤维中天然增强填料的理想候选材料。
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引用次数: 0
Properties of Skin Collagen from Southern Catfish (Silurus meridionalis) Fed with Raw and Cooked Food 南方鲶鱼(Silurus meridionalis)生食和熟食皮肤胶原蛋白的特性
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182901
Qi Zhang, Shufang Hou, Yanmei Liu, Jia Du, Yongkang Jia, Qiushi Yang, Tingting Xu, Yasuaki Takagi, Dapeng Li, Xi Zhang
The southern catfish (Silurus meridionalis) is an economically important carnivorous freshwater fish in China. In this study, we compared the properties of skin collagen from southern catfish fed with raw food (RF) and cooked food (CF). The skin collagen yield in the RF group (8.66 ± 0.11%) was significantly higher than that of the CF group (8.00 ± 0.27%). SDS-PAGE, circular dichroism spectroscopy, and FTIR analyses revealed that the collagen extracted from southern catfish skin in both groups was type I collagen, with a unique triple helix structure and high purity. The thermal denaturation temperature of collagen in the RF group (35.20 ± 0.11 °C) was significantly higher than that of the CF group (34.51 ± 0.25 °C). The DPPH free radical scavenging rates were 68.30 ± 2.41% in the RF collagen and 61.78 ± 3.91% in the CF collagen, which was higher than that found in most fish collagen. Both the RF and CF groups had high ability to form fibrils in vitro. Under the same conditions, the CF group exhibited faster fibril formation and a thicker fibril diameter (p < 0.05). In addition, the RF group exhibited significantly higher expression of col1a1 compared to the CF group. These results indicated that feeding southern catfish raw food contributed to collagen production, and the collagen from these fish may have potential in biomaterial applications.
南方鲇(Silurus meridionalis)是中国经济上重要的肉食性淡水鱼。本研究比较了生食(RF)和熟食(CF)南方鲇鱼皮肤胶原蛋白的特性。生食组的皮肤胶原蛋白产量(8.66 ± 0.11%)明显高于熟食组(8.00 ± 0.27%)。SDS-PAGE、圆二色光谱和傅立叶变换红外光谱分析显示,两组从南方鲶鱼皮中提取的胶原蛋白均为 I 型胶原蛋白,具有独特的三螺旋结构,纯度较高。RF 组胶原蛋白的热变性温度(35.20 ± 0.11 °C)明显高于 CF 组(34.51 ± 0.25 °C)。RF 胶原蛋白的 DPPH 自由基清除率为 68.30 ± 2.41%,CF 胶原蛋白为 61.78 ± 3.91%,高于大多数鱼类胶原蛋白。RF 组和 CF 组在体外形成纤维的能力都很强。在相同条件下,CF 组的纤维形成速度更快,纤维直径更粗(p < 0.05)。此外,RF 组的 col1a1 表达量明显高于 CF 组。这些结果表明,给南方鲶鱼喂食生食有助于胶原蛋白的产生,这些鱼的胶原蛋白可能具有生物材料应用的潜力。
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引用次数: 0
Recent Development in Detection and Control of Psychrotrophic Bacteria in Dairy Production: Ensuring Milk Quality 乳制品生产中精神营养细菌检测与控制的最新进展:确保牛奶质量
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182908
Kidane Yalew, Xiaoyang Pang, Shixin Huang, Shuwen Zhang, Xianchao Yang, Ning Xie, Yunna Wang, Jiaping Lv, Xu Li
Milk is an ideal environment for the growth of microorganisms, especially psychrotrophic bacteria, which can survive under cold conditions and produce heat-resistant enzymes. Psychrotrophic bacteria create the great problem of spoiling milk quality and safety. Several ways that milk might get contaminated by psychrotrophic bacteria include animal health, cowshed hygiene, water quality, feeding strategy, as well as milk collection, processing, etc. Maintaining the quality of raw milk is critically essential in dairy processing, and the dairy sector is still affected by the premature milk deterioration of market-processed products. This review focused on the recent detection and control strategies of psychrotrophic bacteria and emphasizes the significance of advanced sensing methods for early detection. It highlights the ongoing challenges in the dairy industry caused by these microorganisms and discusses future perspectives in enhancing milk quality through innovative rapid detection methods and stringent processing controls. This review advocates for a shift towards more sophisticated on-farm detection technologies and improved control practices to prevent spoilage and economic losses in the dairy sector.
牛奶是微生物生长的理想环境,尤其是精神营养性细菌,它们能在低温条件下生存并产生耐热酶。精神营养性细菌会造成牛奶质量和安全的严重问题。牛奶可能受到精神营养性细菌污染的几种途径包括动物健康、牛舍卫生、水质、饲喂策略以及牛奶收集、加工等。保持原奶质量对乳品加工至关重要,而乳品行业仍受到市场加工产品过早变质的影响。本综述侧重于精神滋养型细菌的最新检测和控制策略,并强调了先进传感方法对早期检测的重要意义。综述强调了这些微生物给乳制品行业带来的持续挑战,并讨论了通过创新的快速检测方法和严格的加工控制来提高牛奶质量的未来前景。本综述主张转向更先进的牧场检测技术和改进的控制方法,以防止乳制品行业的腐败和经济损失。
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引用次数: 0
Volatile Organic Compounds Produced by Bacillus sp. Strain R2 Inhibit Aspergillus flavus Growth In Vitro and in Unhulled Rice R2 菌株产生的挥发性有机化合物可抑制黄曲霉菌在体外和未脱壳水稻中的生长
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182898
Qingyun Wang, Kaige Zhang, Lu Yu, Qinlu Lin, Wenhua Zhou
Volatile organic compounds (VOCs) produced by Bacillus species exhibit biocontrol activity against fungal pathogens of fruits and vegetables. However, research on the effect of VOCs on Aspergillus flavus in stored grains is limited. This study aimed to investigate the effects of VOCs extracted from the strain R2, which was isolated from unhulled rice and identified as Bacillus paramycoides on A. flavus in vitro and unhulled rice. R2 VOCs effectively inhibited conidial germination and the hyphal growth of A. flavus in vitro. Moreover, R2 VOCs reduced the fungal population, aflatoxin B1 (AFB1) levels, and free fatty acid (FFA) value by 90.8%, 67%, and 38.7%, respectively, in unhulled rice. Eighteen R2 VOCs were identified using headspace solid-phase micro-extraction gas chromatography–mass spectrometry, and the individual activity of the VOCs against A. flavus was tested in vitro. Benzaldehyde (Ben) and 3,7-dimethyl-1-octanol (Dmo) showed strong inhibitory activities against A. flavus on PDA plates, with inhibition rates of 100% and 91.2%, respectively, at a concentration of 20 μL/dish. Ben at the concentration of 0.09 mg/mL, Dmo at the concentration of 0.07 mg/mL, or a mixture of both at halved concentrations could reduce the fungal population, AFB1 levels, and FFA content in unhulled rice. Our findings suggest that R2 VOCs are good alternatives to traditional chemical fumigants for suppressing A. flavus in stored grains. However, further research is necessary to establish the optimal fumigation concentration of these two components in unhulled rice. The impact of their residues on grain quality should be explored through sensory evaluation and nutritional analysis, and their safety to the environment and human body should be evaluated through safety assessment.
芽孢杆菌产生的挥发性有机化合物(VOCs)对水果和蔬菜中的真菌病原体具有生物防治活性。然而,有关挥发性有机化合物对贮藏谷物中黄曲霉菌影响的研究还很有限。本研究旨在研究从菌株 R2 中提取的挥发性有机化合物对黄曲霉的体外和未脱壳大米的影响,R2 菌株是从未脱壳大米中分离出来的,被鉴定为副黏液芽孢杆菌(Bacillus paramycoides)。R2 VOCs 在体外能有效抑制黄曲霉的分生孢子萌发和菌丝生长。此外,R2 VOCs 还能减少未脱壳大米中的真菌数量、黄曲霉毒素 B1(AFB1)含量和游离脂肪酸(FFA)值,降幅分别为 90.8%、67% 和 38.7%。利用顶空固相微萃取气相色谱-质谱法鉴定了 18 种 R2 挥发性有机化合物,并在体外测试了这些挥发性有机化合物对黄曲霉菌的个体活性。在 PDA 平板上,苯甲醛(Ben)和 3,7-二甲基-1-辛醇(Dmo)对黄曲霉具有很强的抑制活性,浓度为 20 μL/dish 时,抑制率分别为 100%和 91.2%。浓度为 0.09 毫克/毫升的 Ben、浓度为 0.07 毫克/毫升的 Dmo 或浓度减半的二者混合物可减少未脱壳大米中的真菌数量、AFB1 含量和 FFA 含量。我们的研究结果表明,R2 挥发性有机化合物是传统化学熏蒸剂的良好替代品,可用于抑制储藏谷物中的黄曲霉。不过,有必要进一步研究确定这两种成分在未脱壳大米中的最佳熏蒸浓度。应通过感官评价和营养分析来探讨其残留对谷物品质的影响,并通过安全评估来评价其对环境和人体的安全性。
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引用次数: 0
Exploring the Diversity and Potential Use of Flower-Derived Lactic Acid Bacteria in Plant-Based Fermentation: Insights into Exo-Cellular Polysaccharide Production 探索植物发酵中花卉衍生乳酸菌的多样性和潜在用途:细胞外多糖生产的启示
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182907
Azadeh Khiabani, Hang Xiao, Anders Peter Wätjen, Miguel Tovar, Vera Kuzina Poulsen, Egon Bech Hansen, Claus Heiner Bang-Berthelsen
Isolation of new plant-derived lactic acid bacteria (LAB) is highly prioritized in developing novel starter cultures for plant-based fermentation. This study explores the diversity of LAB in Danish flowers and their potential use for plant-based food fermentation. A total of 46 flower samples under 34 genera were collected for LAB isolation. By introducing an enrichment step, a total of 61 LAB strains were isolated and identified using MALDI-TOF and 16S rRNA sequencing. These strains represent 24 species across 9 genera, predominantly Leuconostoc mesenteroides, Fructobacillus fructosus, Apilactobacillus ozensis, and Apilactobacillus kunkeei. Phenotypic screening for exo-cellular polysaccharide production revealed that 40 strains exhibited sliminess or ropiness on sucrose-containing agar plates. HPLC analysis confirmed that all isolates produced exo-cellular polysaccharides containing glucose, fructose, or galactose as sugar monomers. Therefore, the strains were glucan, fructan, and galactan producers. The suitability of these strains for plant-based fermentation was characterized by using almond, oat, and soy milk. The results showed successful acidification in all three types of plant-based matrices but only observed texture development in soy by Leuconostoc, Weissella, Lactococcus, Apilactobacillus, and Fructobacillus. The findings highlight the potential of flower-derived LAB strains for texture development in soy-based dairy alternatives.
分离新的植物源乳酸菌(LAB)是开发用于植物发酵的新型启动培养物的重中之重。本研究探讨了丹麦花卉中 LAB 的多样性及其用于植物性食品发酵的潜力。本研究共收集了 46 个花卉样本,涉及 34 个属,用于分离 LAB。通过引入富集步骤,共分离出 61 株 LAB,并使用 MALDI-TOF 和 16S rRNA 测序进行了鉴定。这些菌株代表了 9 个菌属中的 24 个菌种,主要是中肠亮菌(Leuconostoc mesenteroides)、果寡糖杆菌(Fructobacillus fructosus)、奥兹杏杆菌(Apilactobacillus ozensis)和昆明杏杆菌(Apilactobacillus kunkeei)。细胞外多糖生产的表型筛选显示,40 株菌株在含蔗糖的琼脂平板上表现出纤细或粗糙。高效液相色谱分析证实,所有分离菌株都能产生以葡萄糖、果糖或半乳糖为糖单体的胞外多糖。因此,这些菌株是葡聚糖、果聚糖和半乳糖的生产者。通过使用杏仁奶、燕麦奶和豆奶对这些菌株是否适合植物发酵进行了鉴定。结果表明,这三种植物基质都能成功酸化,但只观察到大豆中的白色念珠菌、魏氏菌、乳球菌、乳酸杆菌和果酸杆菌产生了质构。研究结果凸显了花源 LAB 菌株在大豆乳制品替代品质构发展中的潜力。
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引用次数: 0
Effects of Dietary Supplementation with Cocrystals of Thymol and Carvacrol on Quality, Nutrient Composition, and Oxidative Stability of Broiler Meat 膳食中添加百里酚和香芹酚胶晶对肉鸡肉质、营养成分和氧化稳定性的影响
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182899
Yang Li, Changjin Li, Yunlong Zhang, Nadia Everaert, Luke Comer, Libo Huang, Ning Jiao, Xuejun Yuan, Weiren Yang, Shuzhen Jiang
Consumer demand for high-quality meat has increased. This study aimed to investigate the potential application of cocrystals of thymol and carvacrol in broilers for high-quality meat production. Eight hundred 1-day-old chicks were assigned to four groups fed diets supplemented with 0, 40, 60, and 80 mg/kg of Crystal EO® (CEO), containing 25% cocrystals of thymol and carvacrol in a 42-d feeding trial. The results showed that dietary CEO supplementation decreased the muscle fiber diameter and increased the muscle fiber density, glycogen content, L*45 min value, and proportion of α-linolenic acid in the breast muscle; dietary 40 and 60 mg/kg of CEO decreased the lactate content, MDA concentration, cooking loss, shear force, and thrombogenicity index and increased the proportion of lauric acid in the breast muscle; dietary 60 and 80 mg/kg of CEO increased the glucose content, total superoxide dismutase, and total antioxidant capacity levels of breast muscle. Citrate synthase activity, free radical scavenging capacity, pH24 h and a*45 min values, and the cystine content in the breast muscle were especially higher in the 60 mg/kg CEO group compared to the control group. Collectively, dietary CEO supplementation improved meat quality and nutritional values and enhanced the antioxidant capacity of broiler meat, with 60 mg/kg of CEO having the greatest effect.
消费者对高品质肉类的需求不断增加。本研究旨在调查百里酚和香芹酚的共晶体在肉鸡优质肉生产中的潜在应用。在为期42天的饲养试验中,800只1日龄雏鸡被分配到4个组,分别饲喂添加0、40、60和80毫克/千克Crystal EO®(CEO)的日粮,其中含有25%的百里酚和香芹酚共晶体。结果表明,膳食中添加CEO可降低胸肌的肌纤维直径,提高胸肌的肌纤维密度、糖原含量、L*45 min值和α-亚麻酸比例;膳食中添加40和60 mg/kg的CEO可降低胸肌的乳酸含量、MDA浓度、蒸煮损失、剪切力和血栓形成指数,提高月桂酸比例;膳食中添加60和80 mg/kg的CEO可提高胸肌的葡萄糖含量、总超氧化物歧化酶和总抗氧化能力水平。与对照组相比,60 毫克/千克 CEO 组的柠檬酸合成酶活性、自由基清除能力、pH24 小时值和 a*45 分钟值以及胸肌中的胱氨酸含量尤其高。总之,膳食中补充 CEO 可改善肉质和营养价值,提高肉鸡肉的抗氧化能力,其中 60 毫克/千克 CEO 的效果最好。
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引用次数: 0
Utilizing Sensory and Visual Data in the Value Estimation of Extra Virgin Olive Oil 利用感官和视觉数据估算特级初榨橄榄油的价值
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182904
Seidi Suurmets, Jesper Clement, Simone Piras, Carla Barlagne, Matilde Tura, Noureddine Mokhtari, Chokri Thabet
Food evaluation is a topic central to consumer research and food marketing. However, there is little consensus regarding how consumers combine sensory stimuli, product information, and visual impressions to shape their evaluation. Moreover, the bulk of research relies on studies based on questionnaires and declarative responses, raising questions about subliminal processes and their hierarchy in an evaluation process. To address this gap in the literature, we conducted a study with more than 400 participants in Morocco and Tunisia and investigated how factors such as flavor/taste, product information, and packaging design in a variety of olive oils influence visual attention and are reflected in willingness to pay (WTP). We implemented incentivization through an auction to reduce the hypothetical bias in stated WTP values. The results revealed that, compared to tasting the oils, the provision of cognitive information led to an increase in consumers’ WTP. However, a drastic increase in WTP occurred when the consumers were exposed to package designs, overshadowing the formerly dominant effects of product attributes. These findings support theories suggesting a visual perceptual processing advantage due to the picture superiority effect–a picture says a thousand words. Further, it underlines the importance of graphic design in food marketing. The findings have ramifications for food marketing, product development, and pricing strategies.
食品评价是消费者研究和食品营销的核心课题。然而,对于消费者如何结合感官刺激、产品信息和视觉印象来形成他们的评价,目前还鲜有共识。此外,大部分研究都是基于问卷调查和陈述性反应进行的,这就对潜意识过程及其在评价过程中的层次提出了疑问。为了填补这一文献空白,我们在摩洛哥和突尼斯对 400 多名参与者进行了一项研究,调查了各种橄榄油的风味/口感、产品信息和包装设计等因素如何影响视觉注意力并反映在支付意愿(WTP)中。我们通过拍卖实施激励措施,以减少 WTP 值中的假设偏差。结果表明,与品尝橄榄油相比,提供认知信息会提高消费者的 WTP。然而,当消费者接触到包装设计时,他们的 WTP 会急剧增加,从而掩盖了之前产品属性的主要影响。这些发现支持了视觉感知处理优势的理论,即图片优势效应--一张图片胜过千言万语。此外,它还强调了平面设计在食品营销中的重要性。这些发现对食品营销、产品开发和定价策略都有影响。
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引用次数: 0
Analytical Investigation of Phthalates and Heavy Metals in Edible Ice from Vending Machines Connected to the Italian Water Supply 意大利供水自动售货机食用冰中邻苯二甲酸盐和重金属的分析调查
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182910
Nicoletta De Vietro, Francesco Triggiano, Pietro Cotugno, Jolanda Palmisani, Alessia Di Gilio, Carlo Zambonin, Gianluigi de Gennaro, Giovanna Mancini, Antonella Maria Aresta, Giusy Diella, Vincenzo Marcotrigiano, Giovanni Trifone Sorrenti, Piersaverio Marzocca, Michele Lampedecchia, Domenico Pio Sorrenti, Ezio D’Aniello, Matilde Gramegna, Alessandra Nencha, Antonio Caputo, Marta Giovine, Caterina Spinelli, Giuseppina Caggiano
Edible ice is often produced by special machines that can represent a source of significant chemical and microbiological contamination. In this work, the presence of phthalic acid esters (phthalates, PAEs) and heavy metals in ice cubes distributed by 77 vending machines installed in two different zones in southern Italy and fed by water from the public water supply was investigated. Solid-phase microextraction coupled to gas chromatography−mass spectrometry (SPME-GC/MS) was used to evaluate contamination with four PAEs, which were selected because they are commonly used in the production of food-contact plastics, while inductively coupled plasma mass spectrometry (ICP/MS) was used to quantify the heavy metals. It was found that ice samples, especially those from one of the two considered zones (zone 2), exceeded the dibutyl phthalate (DBP) threshold limit value; some ice cubes from the other zone (zone 1) instead showed levels of both lead (Pb) and nickel (Ni) up to one order of magnitude higher than those observed in samples collected in zone 2 and higher than the maximum permitted values (European Directive n. 2184/2020). Since the water source connected to the ice vending machines was found to be free from significant levels of all considered target compounds and metals, the high levels of DBP, Ni, and Pb in ice cubes could be attributed to the components and/or to the state of repair of the ice vending machines themselves.
食用冰通常是由特殊机器生产的,这些机器可能是严重的化学和微生物污染源。这项研究调查了意大利南部两个不同地区的 77 台自动售货机分发的冰块中邻苯二甲酸酯(PAEs)和重金属的含量。采用固相微萃取-气相色谱-质谱联用技术(SPME-GC/MS)评估了四种 PAEs 的污染情况,之所以选择这四种 PAEs 是因为它们常用于生产与食品接触的塑料,而采用电感耦合等离子体质谱联用技术(ICP/MS)则对重金属进行了定量。结果发现,冰块样本,尤其是来自两个区域之一(区域 2)的冰块样本,超过了邻苯二甲酸二丁酯(DBP)阈值限值;而来自另一个区域(区域 1)的一些冰块样本显示,铅(Pb)和镍(Ni)的含量比在区域 2 采集的样本中观察到的含量高出一个数量级,并且高于最高允许值(欧洲指令 n.2184/2020)。由于发现连接自动售冰机的水源中没有大量的目标化合物和金属,冰块中较高的 DBP、Ni 和 Pb 含量可能是由于自动售冰机本身的组件和/或维修状态造成的。
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引用次数: 0
An Overview of Interactions between Goat Milk Casein and Other Food Components: Polysaccharides, Polyphenols, and Metal Ions 山羊奶酪蛋白与其他食物成分之间的相互作用概述:多糖、多酚和金属离子
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182903
Bohan Ma, Majida Al-Wraikat, Qin Shu, Xi Yang, Yongfeng Liu
Casein is among the most abundant proteins in milk and has high nutritional value. Casein’s interactions with polysaccharides, polyphenols, and metal ions are important for regulating the functional properties and textural quality of dairy foods. To improve the functional properties of casein-based foods, a deep understanding of the interaction mechanisms and the influencing factors between casein and other food components is required. This review started by elucidating the interaction mechanism of casein with polysaccharides, polyphenols, and metal ions. Thermodynamic incompatibility and attraction are the fundamental factors in determining the interaction types between casein and polysaccharides, which leads to different phase behaviors and microstructural types in casein-based foods. Additionally, the interaction of casein with polyphenols primarily occurs through non-covalent (hydrogen bonding, hydrophobic interactions, van der Waals forces, and ionic bonding) or covalent interaction (primarily based on the oxidation of proteins or polyphenols by enzymatic or non-enzymatic (alkaline or free radical grafting) approaches). Moreover, the selectivity of casein to specific metal ions is also introduced. Factors affecting the binding of casein to the above three components, such as temperature, pH, the mixing ratio, and the fine structure of these components, are also summarized to provide a good foundation for casein-based food applications.
酪蛋白是牛奶中含量最高的蛋白质之一,具有很高的营养价值。酪蛋白与多糖、多酚和金属离子的相互作用对于调节乳制品食品的功能特性和质构质量非常重要。为了改善酪蛋白食品的功能特性,需要深入了解酪蛋白与其他食品成分之间的相互作用机制和影响因素。本综述首先阐明了酪蛋白与多糖、多酚和金属离子的相互作用机理。热力学不相容性和吸引力是决定酪蛋白与多糖之间相互作用类型的基本因素,这导致了酪蛋白食品中不同的相行为和微观结构类型。此外,酪蛋白与多酚的相互作用主要是通过非共价(氢键、疏水作用、范德华力和离子键)或共价(主要是通过酶或非酶(碱性或自由基接枝)方法使蛋白质或多酚氧化)发生的。此外,还介绍了酪蛋白对特定金属离子的选择性。此外,还总结了影响酪蛋白与上述三种成分结合的因素,如温度、pH 值、混合比以及这些成分的精细结构,从而为酪蛋白食品的应用奠定了良好的基础。
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引用次数: 0
Potential Skin Health Benefits of Abalone By-Products Suggested by Their Effects on MAPKS and PI3K/AKT/NF-kB Signaling Pathways in HDF and HaCaT Cells 鲍鱼副产品对 HDF 和 HaCaT 细胞中 MAPKS 和 PI3K/AKT/NF-kB 信号通路的影响揭示了鲍鱼副产品对皮肤健康的潜在益处
IF 5.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-13 DOI: 10.3390/foods13182902
Eun-A Kim, Nalae Kang, Jun-Ho Heo, Areumi Park, Seong-Yeong Heo, Chang-Ik Ko, Yong-Seok Ahn, Ginnae Ahn, Soo-Jin Heo
Abalone, a marine edible gastropod with nutritional value, is a popular seafood delicacy worldwide, especially in Asia; however, viscera by-products are generally discarded during processing. Therefore, we investigated the skin health benefits of abalone viscera ultrasonic extract (AVU) in human dermal fibroblasts (HDFs) and human keratinocyte (HaCaT) cells. AVU showed valuable protein contents, indicating that it is a worthy and safe material for industrial application. AVU increased collagen synthesis production and messenger RNA (mRNA) expression of Collagen Type I Alpha 1, 2, and 3 chains through the transforming growth factor beta/suppressor of mother against the decapentaplegic pathway in HDF cells. AVU also increased hyaluronic acid production, upregulated Hyaluronan Synthases 1, 2, and 3, filaggrin and aquaporin3 mRNA levels, and downregulated hyaluronidase mRNA levels in HaCaT cells. Furthermore, mechanistic studies showed that AVU increased the phosphorylation of extracellular signal-regulated kinase, p38, and cyclic AMP response-binding protein activation. AVU activated the transcription factors, phosphoinositide 3-kinase, protein kinase B, and nuclear factor kappa B cell p65 and downregulated the degranulation of inhibitory kappa B in HaCaT cells. Studies of hyaluronic acid production in AVU by inhibiting EKR, p38 and NF-κB have shown that p38 MAPK and NF-κB signaling are pivotal mechanisms, particularly in the AVU. These results demonstrated that AVU produced from by-products may improve skin health and may thus be used as a functional food and cosmetics ingredient.
鲍鱼是一种具有营养价值的海洋食用腹足类动物,是世界上尤其是亚洲地区广受欢迎的海鲜美食;然而,鲍鱼内脏副产品在加工过程中通常会被丢弃。因此,我们研究了鲍鱼内脏超声提取物(AVU)对人类真皮成纤维细胞(HDFs)和人类角质细胞(HaCaT)皮肤健康的益处。鲍鱼内脏超声提取物显示出珍贵的蛋白质含量,表明它是一种值得工业应用的安全材料。AVU 可通过转化生长因子 beta/母体抗衰老抑制因子途径,增加 HDF 细胞中胶原蛋白的合成和 I 型胶原蛋白 Alpha 1、2 和 3 链的信使 RNA(mRNA)表达。AVU 还能增加 HaCaT 细胞中透明质酸的产生,上调透明质酸合成酶 1、2 和 3、filaggrin 和 aquaporin3 mRNA 水平,下调透明质酸酶 mRNA 水平。此外,机理研究表明,AVU 增加了细胞外信号调节激酶、p38 的磷酸化和环 AMP 反应结合蛋白的活化。AVU 激活了转录因子磷酸肌酸 3-激酶、蛋白激酶 B 和核因子卡巴 B 细胞 p65,并下调了 HaCaT 细胞中抑制性卡巴 B 的脱颗粒作用。通过抑制 EKR、p38 和 NF-κB 在 AVU 中产生透明质酸的研究表明,p38 MAPK 和 NF-κB 信号转导是关键机制,尤其是在 AVU 中。这些结果表明,从副产品中提取的 AVU 可改善皮肤健康,因此可用作功能性食品和化妆品成分。
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