Long-term monitoring of biofilm succession unveils differences between biodegradable and conventional plastic materials

IF 5.3 3区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Marine pollution bulletin Pub Date : 2025-03-15 DOI:10.1016/j.marpolbul.2025.117820
Anna Marín , Patricia Feijóo , Belén Carbonetto , Pedro González-Torres , José Tena-Medialdea , José R. García-March , José Gámez-Pérez , Luis Cabedo
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引用次数: 0

Abstract

A vast amount of plastic waste enters the ocean every year and the Mediterranean Sea is particularly affected by this issue. Biodegradable polymers like poly(lactic acid) (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), may help mitigate this problem. We investigated bacterial biofilm development and succession on these polymers over one year in the Western Mediterranean Sea. Scanning electron microscopy (SEM) and confocal laser scanning were used to examine microbial colonization and surface erosion, while bacterial community abundance and composition were assessed through culture plate counting and 16S rRNA gene amplicon sequencing. SEM revealed significant surface erosion on PHBV, indicative of microbial degradation, while PLA exhibited minor and irregular erosion. Culture-based quantification showed higher bacterial colonization on PHBV compared to PLA, suggesting that PHBV provides a more favourable surface for bacterial attachment Amplicon sequencing of the 16S rRNA gene revealed high bacterial diversity, with 17,781 operational taxonomic units across all samples. Proteobacteria, Bacteroidota, and Planctomycetota were the dominant phyla, with the Shannon index consistently exceeding 8, corroborating the bacterial diversity across all materials. Temporal shifts in bacterial community composition were significant, with exposure time explaining 29.8 % of the variation, suggesting biofilm succession as a key factor shaping microbial assemblages. While polymer type had a limited impact on bacterial composition, PHBV biofilms exhibited greater bacterial abundance and diversity compared to PLA. This study highlights PHBV's role in shaping biofilms and its relevance in assessing biodegradable plastics in marine environments. Understanding microbial interactions with bioplastics is crucial for evaluating their environmental impact and degradation dynamics.
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来源期刊
Marine pollution bulletin
Marine pollution bulletin 环境科学-海洋与淡水生物学
CiteScore
10.20
自引率
15.50%
发文量
1077
审稿时长
68 days
期刊介绍: Marine Pollution Bulletin is concerned with the rational use of maritime and marine resources in estuaries, the seas and oceans, as well as with documenting marine pollution and introducing new forms of measurement and analysis. A wide range of topics are discussed as news, comment, reviews and research reports, not only on effluent disposal and pollution control, but also on the management, economic aspects and protection of the marine environment in general.
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