Marine pollution bulletin最新文献

{"title":"Effect of noise on the behaviour and microbiome of a common temperate estuarine fish","authors":"Brittney Valenzisi ,&nbsp;Troy F. Gaston ,&nbsp;Miles Parsons ,&nbsp;Megan J. Huggett","doi":"10.1016/j.marpolbul.2026.119347","DOIUrl":"10.1016/j.marpolbul.2026.119347","url":null,"abstract":"<div><div>Underwater noise pollution is an increasing stressor in marine and estuarine environments, with anthropogenic activity altering natural soundscapes and adversely affecting animal behaviour, which is often an early indicator of environmental stress with broader physiological implications. Host-associated microbiomes, particularly the gut, are central to organism health, influencing digestion, immunity, metabolism, and stress regulation. The gut microbiome also plays a key role in linking environmental disturbances with physiological condition and behavioural changes, yet the impacts of noise on these microbial communities remain poorly understood. We examined the behavioural and gut microbial response of <em>Pelates sexlineatus</em> exposed to three acoustic treatments: continuous white noise (i.e. urban hum), short-pulsed white noise (i.e. intermittent boat noise), and ambient control (no added noise), under controlled aquarium conditions. Fish in ambient tanks spent the most time swimming, while fish exposed to noise exhibited increased resting and erratic movements. Microbial communities in fish and water samples, characterised using 16S rRNA gene amplicon sequencing, identified 44 phyla in the fish microbiome and 11 comprised the core microbiome. Strong differentiation was observed between fish and water communities with fish dominated by <em>Firmicutes</em>, <em>Proteobacteria</em> and <em>Actinobacteriota,</em> and water dominated by <em>Bacteroidota</em> and <em>Proteobacteria</em>. Although short-term acoustic exposure did not significantly alter overall microbial diversity, subtle compositional shifts and strong host-environment distinctions highlight the complexity of microbiome responses to environmental stress. This study provides novel insights into the short-term stability of the gut microbiome in estuarine fish and highlights the need for integrated research assessing behavioural and microbial responses to noise pollution.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119347"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable isotopes δD–H2O, δ18O–H2O, δ15N–NO3−, δ18O–NO3− and hydrochemistry of the volcanic catchments and the influence of continental runoff on the environment of Eastern Kamchatka","authors":"Pavel Semkin ,&nbsp;Galina Pavlova ,&nbsp;Shan Jiang ,&nbsp;Olga Ulanova ,&nbsp;Yuri Barabanshchikov ,&nbsp;Maria Shvetsova ,&nbsp;Anna Kozachek ,&nbsp;Yi Xu ,&nbsp;Jing Zhang ,&nbsp;Vyacheslav Lobanov","doi":"10.1016/j.marpolbul.2026.119323","DOIUrl":"10.1016/j.marpolbul.2026.119323","url":null,"abstract":"<div><div>Volcanic catchments and coastal areas near active volcanoes have specific ecosystems in many regions of the world primarily because of availability of nutrients (N, P, Si) and trace elements supplied from volcanism. Isotopic (δD–H₂O, δ¹⁸O–H₂O, δ¹⁵N–<span><math><msubsup><mi>NO</mi><mn>3</mn><mo>−</mo></msubsup></math></span>, δ¹⁸O–<span><math><msubsup><mi>NO</mi><mn>3</mn><mo>−</mo></msubsup></math></span>) and hydrochemical data from eight different rivers and results of three cruises on both sides of the Kamchatka Peninsula held from 2022 to 2025 together with satellite data of chlorophyll “a” concentration are discussed in this article. Unlike other volcanic regions of the world, the Eastern Kamchatka catchments have a lengthy spring-summer flooding as a result of snow melting. Seasonal effect of dilution in coastal Pacific waters is identified at the depths of up to 500 m and at the distances of &gt;150 km from the shore line. In the period of increasing discharge of volcanic rivers of Eastern Kamchatka, concentrations of DIN and DIP nutrients systematically increase because accumulation in the snow is observed during volcanism. As a result, with spring-summer stratification of coastal Pacific waters, phytoplankton blooms occur from May to July and this is having good correlation with the period of high-water discharge of the rivers. Next bloom stage, which often involves the harmful dinoflagellates and sometimes large-scale “red tides”, occurs in September/October. Our hypothesis for this bloom stage is significant increase of DIN/DIP ratio in years with hot summer. This occurs due to increased concentrations of DIN in the snow near the top of volcanos, which accumulates during the previous winter due to volcanic exhalations. It is explained that snow melting process at the highlands supply additional DIN to the coastal part of the Pacific Ocean. Thus, the most intense phytoplankton bloom in the adjacent part of the Pacific Ocean should be expected in the years with both a large amount of snow in the previous winter and sufficiently high air temperatures in summer for intense snow melting in the highlands and the formation of stratification of the coastal Pacific waters.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119323"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mobilization of cadmium and lead mediated by iron and manganese reduction in coastal wetlands of Yueqing Bay, China","authors":"Qing-Rui Jiang ,&nbsp;Jia-Xi Pang ,&nbsp;Wei-Wei Ma ,&nbsp;Wen-Zhuo Zhu ,&nbsp;Jian Wang ,&nbsp;Xiao-Ping Chai ,&nbsp;Lu-Ping Song ,&nbsp;Yi-Ying Zhang ,&nbsp;Jian-Xin Wang","doi":"10.1016/j.marpolbul.2026.119285","DOIUrl":"10.1016/j.marpolbul.2026.119285","url":null,"abstract":"<div><div>The mobilization of heavy metals (HMs) in coastal wetland sediments presents considerable ecological risks to the adjacent sea area, yet the key processes controlling their transport and transformation are not well understood. This study employed sequential extraction to analyze the chemical speciation of Fe, Mn, and six HMs (Cu, Ni, Cr, Pb, Zn, and Cd) in the coastal wetlands of Yueqing Bay, aiming to evaluate the influence of Fe and Mn geochemical processes on the ecological risks of HMs. Speciation analysis indicated that Cd exhibits higher bioavailability, primarily associated with the exchangeable and carbonate-bound fractions (F1-Cd, 26.0%). In contrast, Pb was predominantly enriched in the organically complexed fraction (F3-Pb, 48.0%), implying a potential release risk during organic matter mineralization. The remaining studied HMs were primarily present within the residual fraction with Cr (93.6%) &gt; Zn (85.5%) &gt; Ni (84.3%) &gt; Cu (80.4%), indicating a dominant natural lithogenic source. The prevalence of Mn(II)‑carbonates (66.4% of Mn_T) indicates widespread Mn reduction, which is identified as a primary mechanism for the enrichment of bioavailable Cd in the F1 fraction. The partition of Pb speciation is co-influenced by Mn and Fe oxides, whose reductive dissolution releases Pb, subsequently facilitating its complexation with organic matter into F3-Pb. These results highlight the crucial role of Mn and Fe redox cycling in controlling HM mobilization and speciation in coastal wetland sediments.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119285"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benthic–pelagic interactions and algal-derived organic matter dynamics along the East Siberian Sea slope revealed by fluorescence and isotope tracers","authors":"Silpa Mathew ,&nbsp;Jong Kuk Hong ,&nbsp;Tae Siek Rhee ,&nbsp;Kyung-Hoon Shin ,&nbsp;Seeryang Seong ,&nbsp;Ji-Hoon Kim ,&nbsp;Meilian Chen ,&nbsp;Jin Hur","doi":"10.1016/j.marpolbul.2026.119334","DOIUrl":"10.1016/j.marpolbul.2026.119334","url":null,"abstract":"<div><div>The Arctic Ocean is undergoing rapid transformation driven by climate change, including declining sea-ice cover and increasing freshwater inputs, with profound consequences for carbon and nutrient cycling. The East Siberian Sea (ESS), one of the largest Arctic shelf systems, plays a central role in linking terrestrial inputs, primary production, and deep-ocean carbon export. In this study, we combined fluorescence spectroscopy with stable isotope and bulk chemical analyses to examine dissolved organic matter (DOM) dynamics along a shelf-slope transect of the ESS. Our results show that slope-associated sedimentary processes exert a strong control on DOM redistribution and transformation. Enhanced algal production across the Russian Arctic increasingly contributes to organic matter transport toward slope regions. Elevated chlorophyll-a concentrations in waters, together with a pronounced enrichment of protein-like fluorescent DOM (C3), point to strong algal inputs to the continental slope. At mid-slope stations (200–900 m), co-enrichment of dissolved and particulate organic carbon with heavier δ¹⁵N signatures suggests intensified microbial remineralization and nitrogen cycling, likely stimulated by sediment resuspension under Atlantic Water influence. These findings highlight the ESS slope as a dynamic benthic–pelagic interface where sedimentary processes reshape DOM composition and act as an important pathway for the supply of bioavailable carbon and nitrogen to the Arctic Ocean interior. Overall, this study highlights the continental slope as an active regulator of Arctic DOM cycling under ongoing Atlantification and sea-ice decline.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119334"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine oil film detection method based on growing hierarchical neural gas network and multi-scale threshold segmentation","authors":"Baozhu Jia ,&nbsp;Zekun Guo ,&nbsp;Jin Xu ,&nbsp;Bo Li ,&nbsp;Yuanyuan Huang ,&nbsp;Min Cheng ,&nbsp;Bo Xu ,&nbsp;Boxi Yao ,&nbsp;Peng Liu","doi":"10.1016/j.marpolbul.2026.119337","DOIUrl":"10.1016/j.marpolbul.2026.119337","url":null,"abstract":"<div><div>The increasement of offshore oil extraction and transportation has brought increasingly severe oil spill risks, seriously endangering the marine ecological environment. Therefore, it is urgent to develop advanced and reliable oil film monitoring technology. An oil film detection method is proposed based on the Growing Hierarchical Neural Gas Network (GHNG) and multi-scale threshold segmentation to address the problem of weak oil film region features and severe environmental noise interference in marine radar images. This method first utilizes the unsupervised learning characteristics of GHNG network to perform dynamic topology learning and hierarchical clustering on preprocessed radar images, effectively distinguishing oil film regions from background interference. Subsequently, a multi-scale adaptive threshold segmentation technique was adopted to accurately extract oil film targets by fusing local thresholds from different scales and neighborhoods. Finally, the final segmentation result is obtained through noise filtering and coordinate transformation. The experimental results indicate that the proposed method provides an effective technical solution for automatic and precise monitoring of marine oil films under complex sea conditions.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119337"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fate of deep methane bubble plumes at the Haima cold seeps in the South China Sea","authors":"Binbin Guo ,&nbsp;Danyi Su ,&nbsp;Shengxiong Yang ,&nbsp;Andi Xu ,&nbsp;Xianhai Bu ,&nbsp;Zongheng Chen ,&nbsp;Xuemin Wu ,&nbsp;Qiangqiang Feng ,&nbsp;Chunliang Chen ,&nbsp;Feng Wang ,&nbsp;Yang Wu ,&nbsp;Yifei Dong ,&nbsp;Xi Xiao ,&nbsp;Qianyong Liang","doi":"10.1016/j.marpolbul.2026.119308","DOIUrl":"10.1016/j.marpolbul.2026.119308","url":null,"abstract":"<div><div>Submarine methane seepage constitutes the primary pathway for carbon transfer from Earth's subsurface to the ocean. This study investigates the fate of deep methane bubble plumes at the Haima cold seeps (1408–1522 m depth)—the largest active seep area in the South China Sea (SCS)—through integrated field observations and numerical simulations. Methane bubbles, encased by hydrate shells, ascended at velocities of 11.1–33.4 cm/s near the seabed. Local hydrographic conditions generated distinct plume morphologies: a sinuous flare at seep HM01 and an eastward-tilted conical plume at seep HMS18, with maximum observed heights of 797 m and widths of 244 m. Plumes terminated near the hydrate stability zone (HSZ) upper boundary, which oscillated between 580 and 645 m depth due to temperature modulation by mesoscale eddies (vertical amplitude: 32.5 m) and tides (5.6 m). Enhanced currents or weakened eruptions can intermittently suppress plume below this boundary. Nine-month moored current observations constrained the maximum lateral bubble displacement to 727 m. Dissolved methane reached 5.3 μM at seabed vents but declined by 1–2 orders of magnitude within 20–40 m above the vents, demonstrating that high-concentration anomalies due to dissolved-phase emissions were trapped near the seabed by density stratification. Numerical simulations predicted a secondary methane concentration anomaly (1.5 nM increase) at the HSZ upper boundary, resulting from hydrate shell dissociation of big bubbles. This study provides critical insights for designing methane leakage monitoring strategies during natural gas hydrate exploitation in the SCS.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119308"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146071833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International maritime organization, we may have a problem - ballast water treatment triggers global concerns with antimicrobial resistant bacteria","authors":"Jan David ,&nbsp;Stephan Gollasch ,&nbsp;Matej David","doi":"10.1016/j.marpolbul.2026.119277","DOIUrl":"10.1016/j.marpolbul.2026.119277","url":null,"abstract":"<div><div>Vessels sailing worldwide need to treat ballast water to inactivate organisms to comply with the Ballast Water Management Convention. We analysed the phenotypic resistance of isolated unidentified bacteria to antimicrobial substances in the uptake water before treatment and the discharged water after treatment on a vessel in operation with a certified UV based ballast water management system (BWMS). We isolated <em>Escherichia coli</em> on selective media and genotyped the identified isolates. All isolates were categorized into clonal and phylogenetic groups based on Polymerase Chain Reaction (PCR) methods. We examined the presence of Plasmid-mediated Quinolone Resistance (PMQR) genes, betalactamases, adhesins, autotransporters, genes encoding iron uptake proteins, genes associated with immune system evasion, and genotoxins. For all non-clonal isolates, we tested their sensitivity to antimicrobial substances. Resistance to cefotaxime increased after treatment in both intrinsic resistance and <em>E. coli</em> isolates. PCR analyses showed a decrease in the occurrence of <em>qnrS</em> and <em>ompT</em>_APEC genes in the discharged water, and an increase in <em>qnrB</em>, <em>kps</em>_MTII, <em>bla</em>_TEM, <em>fyuA</em>, and <em>usp</em> genes. The results of the disk diffusion method indicated an increase in resistance to ampicillin, ceftazidime, and the highest increase occurred in resistance to cefotaxime. The number of multi-drug resistant isolates decreased substantially in the discharged water. BWMS effectively eliminate plankton, while UV water treatment causes different negative changes in bacteria, introducing new threats to human health (microbiome to accumulate resistance genes) and aquaculture (increase diversity and volume of antibiotics used, lower seafood production), hence opening a new issue in global discharges of ballast water.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119277"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of seasonal variations in energy sources and trophic positions of Calanus sinicus and Euphausia pacifica in the central-eastern Yellow Sea using bulk and compound-specific stable isotope analyses","authors":"Jiseung Kang ,&nbsp;Yeonjung Lee ,&nbsp;Dong Han Choi ,&nbsp;Jae Hoon Noh ,&nbsp;Howon Lee ,&nbsp;Hyung-Ku Kang ,&nbsp;Garam Kim ,&nbsp;Kongtae Ra ,&nbsp;Myung Jin Hyun ,&nbsp;Won Seok Yang ,&nbsp;JongSeok Won ,&nbsp;Bohyung Choi ,&nbsp;Ha-Eun Cho ,&nbsp;Kyung-Hoon Shin","doi":"10.1016/j.marpolbul.2026.119311","DOIUrl":"10.1016/j.marpolbul.2026.119311","url":null,"abstract":"<div><div>A comprehensive understanding of energy pathways sustaining zooplankton that support abundant fishery resources of the Yellow Sea is essential for elucidating ecosystem functioning and informing management strategies. However, knowledge of the trophic dynamics of major zooplankton species in the Yellow Sea remains limited. To investigate the energy sources and trophic positions (TPs) of the copepod <em>Calanus sinicus</em> and the krill <em>Euphausia pacifica</em>, two dominant species in the central-eastern Yellow Sea, we analyzed stable carbon and nitrogen isotope ratios in bulk tissue as well as compound-specific nitrogen isotope analysis of amino acids (CSIA-AA). Samples were collected in August 2020, May and November 2021, and March 2022 to assess seasonal variability. Bulk stable isotope analysis revealed that the TPs of the two species were generally similar, with no apparent differences observed across sampling periods, although some differences in energy sources were observed during spring and at coastal stations. In contrast, the TPs estimated from a CSIA-AA revealed relatively lower values for <em>E. pacifica</em>. Both species exhibited relatively low TPs in spring (∼2), increasing to nearly 3 in winter. Furthermore, the proportion of energy transferred through the microbial food web was lower in <em>E. pacifica</em> than in <em>C. sinicus</em>. In addition, a markedly depleted nitrogen isotopic composition in primary producers was observed during the winter when nitrate concentrations were sufficiently high. These findings demonstrate that CSIA-AA is a powerful tool for detecting subtle seasonal and interspecific differences in the trophic dynamics of zooplankton—key intermediaries linking lower trophic levels to fishery resources.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119311"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy, microbial disruption, and algal toxicity of commercial antifouling coatings: A multi-level assessment","authors":"Emilie Adouane ,&nbsp;Lena Granhag ,&nbsp;Camille Ferré ,&nbsp;Raphaël Lami ,&nbsp;Carole Veckerlé ,&nbsp;Renaud Vuillemin ,&nbsp;Erik Ytreberg","doi":"10.1016/j.marpolbul.2026.119303","DOIUrl":"10.1016/j.marpolbul.2026.119303","url":null,"abstract":"<div><div>To mitigate marine biofouling, copper- and zinc-based antifouling (AF) paints are widely used, although their severe environmental toxicity is well established. Silicone-based fouling-release coatings (FRCs) have emerged as alternatives that prevent adhesion through surface properties rather than biocidal activity. However, their effectiveness and ecological impact across marine environments remain insufficiently assessed. This study compared the performance and environmental effects of five commercial coatings: two biocidal, two FRCs, and one inert coating. Panels were statically exposed for seven months at five European sites spanning salinity and temperature gradients. Fouling development was monitored monthly, and coating leachates were tested on red macroalgae and bacteria. Ecotoxicological assays included growth inhibition of <em>Ceramium tenuicorne</em>, <em>Aliivibrio fischeri</em> bioluminescence, <em>Escherichia coli</em> stress biosensors, quorum-sensing (QS) assays, and biofilm formation of marine bacteria.</div><div>Field experiments showed that FRCs consistently outperformed copper-based coatings in efficacy toward biofouling, even under static conditions. Copper-based leachates were highly toxic to <em>C. tenuicorne</em> (EC₅₀ ≈ 0.46%), whereas FRC leachates showed minimal effects and were about 100 times less toxic. Microbial assays revealed that all coatings—including the biocide-free formulations—altered microbial physiology and behavior: leachates induced protein-damage responses, and both QS signaling and biofilm formation were species-specific, confirming that biocide-free does not mean biologically neutral. These results demonstrate that antifouling leachates act not only as toxicants but also as chemical cues shaping microbial communication and early colonization. Integrating microbial-level responses into antifouling evaluations is therefore essential. Overall, FRCs remain the most environmentally acceptable option, combining strong antifouling performance with minimal toxicity.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119303"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and whole-genome sequencing of antibiotic-resistant bacteria revealed the reservoir for indigenous antibiotic resistance genes in the deepest ocean sediment of the Challenger Deep","authors":"Wenmian Ding ,&nbsp;Yuepeng Wang ,&nbsp;Yuewei Ma ,&nbsp;Ping Chen ,&nbsp;Ji Yang ,&nbsp;Zhiping Song ,&nbsp;Yuguo Wang ,&nbsp;Wenju Zhang ,&nbsp;Xuan Li ,&nbsp;Yanyan Huang ,&nbsp;Peng Nan","doi":"10.1016/j.marpolbul.2025.119206","DOIUrl":"10.1016/j.marpolbul.2025.119206","url":null,"abstract":"<div><div>Understanding the occurrence of antibiotic-resistant bacteria (ARB) and associated antibiotic resistance genes (ARGs) in remote marine environments is crucial for accessing treats of ARG pollution on a border ecological scale. While most studies focused on anthropogenically disturbed settings, the Challenger Deep, as the deepest ocean habitat, offers a unique opportunity to investigate minimally disturbed resistomes. We revived 123 bacterial isolates from the Challenger Deep sediment, assessed their antibiotic susceptibility, and identified their taxonomy via 16S rRNA gene sequencing. Among them, 96 strains (78.0%) were resistant to at least one antibiotic, with high prevalence observed in <em>Halomonas</em>, <em>Idiomarina</em>, <em>Flagellimonas</em>, and <em>Microbacterium</em>. Resistance was most common to ampicillin (73.2%), followed by sulfadiazine (30.1%) and nalidixic acid (4.9%). Untargeted metabolomics identified 359 metabolites in the sediment sample, including 6-aminopenicillanic acid, suggesting local microbial antibiotic production and selective pressure of resistance. Anthropogenic contaminants like nalidixic acid were also detected. Whole-genome sequencing of eight representative ARB strains revealed 77 copies of 26 ARG subtypes, predominantly associated with multidrug resistance and efflux pump mechanisms. Notably, no mobile genetic elements were linked to ARGs, indicating limited horizontal gene transfer. Phylogenetic analyses showed host species specificity of ARGs, independent of geography or environmental context, supporting vertical inheritance from ancestral lineages. This study offers the first culture-based evidence of ARB and ARGs in the Challenger Deep, suggesting that resistance may represent an adaptive trait to extreme conditions and underscoring its ancient, intrinsic origin. Our findings provide critical implications for understanding the revolution and dissemination of resistance in deep-sea environments.</div></div>","PeriodicalId":18215,"journal":{"name":"Marine pollution bulletin","volume":"226 ","pages":"Article 119206"},"PeriodicalIF":4.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}