Dual-mode sensors based on double enzyme-mimicking MnCu nanoflowers as fluorescence probe for the detection of organophosphorus pesticides and 2,4-dichlorophenol

IF 5.4 2区 医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-11 DOI:10.1016/j.colsurfb.2025.114608
Ziyu Yan , Ziwen Liao , Zhengyue Xiao , Yining Zhang , Huajie Peng , Jiali Zhong , Zhuo Li , Peng Xu , Ping Qiu
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Abstract

Organophosphorus pesticides (OPs) and 2,4-dichlorophenol (2,4-DP) are widely used, but their residues have brought serious threatens to environment and human health as environmental pollutants. We synthesized double enzyme-mimicking and multifunctional MnCu flower-like nanoenzymes (NH2-MnCu NFs) with fluorescence properties, oxidase (OXD)-like activity and laccase-like activity. NH2-MnCu NFs exhibit excitation-independent emission fluorescence properties, as well as excellent OXD-like activity (Km = 0.230 mM, Vmax = 2.46 × 10−6 M/s) and laccase-like activity (Km = 0.186 mM, Vmax = 3.46 × 10−2 mM/min). The mechanism of 2,3-diaminophenazine (DAP)-induced quenching of the fluorescence of NH2-MnCu NFs was thoroughly investigated, revealing that it results from the combined effects of the internal filtering effect (IFE), dynamic quenching effect (DQE), and static quenching effect (SQE). It was found that -SH could disrupt the OXD-like activity of NH2-MnCu NFs according to morphological and structural perspectives using SEM and in situ Raman spectroscopy. Based on OXD-like activity, ratiometric fluorescence and colorimetric dual-mode sensor was constructed for the detection of OPs. Additionally a colorimetric fluorescence dual-mode sensor was proposed for the specific detection of 2,4-DP with the use of laccase-like activity of the material. Two sensors were constructed using a single type of nanoenzyme, leveraging their dual-enzyme activities for the detection of OPs and 2,4-DP, respectively. This approach expands the potential applications of nanoenzymes and provides versatile scenarios for their multifunctional use.
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来源期刊
Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces 生物-材料科学:生物材料
CiteScore
11.10
自引率
3.40%
发文量
730
审稿时长
42 days
期刊介绍: Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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