{"title":"A Simple, Rapid, Cost-effective and Reliable Molecular Technique for Early Sex Determination in Phoenix dactylifera.","authors":"Asmita Detroja, Jaykumar Koradiya, Munir Ibrahim, Avani Bhimani, Tirth Chetankumar Bhatt, Gaurav Sanghvi, Ashok Kumar Bishoyi","doi":"10.1016/j.ab.2025.115843","DOIUrl":null,"url":null,"abstract":"<p><p>The Phoenix dactylifera is an important horticultural cash crop in arid and semi-arid regions, known for its sweet, edible, nutritious fruit. Date palm is a diecious plant; females are commercially important to produce fruits, and males are required for pollination. Dates are easily segregated through seeds, then seed-raised plants revealed 50% of each male and female plant. But a farmer needs a 95:5 ratio of female to male in the farming land. However, the gender of the plant can be identified four to five years after planting when it begins to flower, and there is no other method available for the identification of the gender. Hence, the present investigation focused on the development of techniques for the early sex determination of dates. Seventy-nine ISSR primers were used to analyze DNA from bulked female and male date palms. Among 79 markers, one marker, ISSR-49, has been selected and successfully validated in 105 female and 103 male date palm samples. The novel reliable biomarker (TG)8RC alone generates both female (400bp) and male (600bp) specific, unique amplicons. In conclusion, this investigation developed a simple, efficient, cost-effective technique that can discriminate the gender of date palms in a single PCR reaction within 5 hours.</p>","PeriodicalId":7830,"journal":{"name":"Analytical biochemistry","volume":" ","pages":"115843"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.ab.2025.115843","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
The Phoenix dactylifera is an important horticultural cash crop in arid and semi-arid regions, known for its sweet, edible, nutritious fruit. Date palm is a diecious plant; females are commercially important to produce fruits, and males are required for pollination. Dates are easily segregated through seeds, then seed-raised plants revealed 50% of each male and female plant. But a farmer needs a 95:5 ratio of female to male in the farming land. However, the gender of the plant can be identified four to five years after planting when it begins to flower, and there is no other method available for the identification of the gender. Hence, the present investigation focused on the development of techniques for the early sex determination of dates. Seventy-nine ISSR primers were used to analyze DNA from bulked female and male date palms. Among 79 markers, one marker, ISSR-49, has been selected and successfully validated in 105 female and 103 male date palm samples. The novel reliable biomarker (TG)8RC alone generates both female (400bp) and male (600bp) specific, unique amplicons. In conclusion, this investigation developed a simple, efficient, cost-effective technique that can discriminate the gender of date palms in a single PCR reaction within 5 hours.
期刊介绍:
The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field.
The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology.
The journal has been particularly active in:
-Analytical techniques for biological molecules-
Aptamer selection and utilization-
Biosensors-
Chromatography-
Cloning, sequencing and mutagenesis-
Electrochemical methods-
Electrophoresis-
Enzyme characterization methods-
Immunological approaches-
Mass spectrometry of proteins and nucleic acids-
Metabolomics-
Nano level techniques-
Optical spectroscopy in all its forms.
The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.