Ameliorative potential of Populus alba leaf powder against hexaflumuron exposure in Nile tilapia: immune-antioxidant, biochemical, histological, and transcriptomic analysis.

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fish Physiology and Biochemistry Pub Date : 2025-03-14 DOI:10.1007/s10695-025-01465-3
Rowida E Ibrahim, Mohamed F M Farag, Mohammed S Sobh, Abdelwahab A Abdelwarith, Elsayed M Younis, Shefaa M Bazeed, Aya Elgamal, Tarek Khamis, Simon J Davies, Afaf N Abdel Rahman
{"title":"Ameliorative potential of Populus alba leaf powder against hexaflumuron exposure in Nile tilapia: immune-antioxidant, biochemical, histological, and transcriptomic analysis.","authors":"Rowida E Ibrahim, Mohamed F M Farag, Mohammed S Sobh, Abdelwahab A Abdelwarith, Elsayed M Younis, Shefaa M Bazeed, Aya Elgamal, Tarek Khamis, Simon J Davies, Afaf N Abdel Rahman","doi":"10.1007/s10695-025-01465-3","DOIUrl":null,"url":null,"abstract":"<p><p>Contamination of the aquatic bodies with pesticides is a serious issue that hinders the aquaculture industry worldwide. Preventing aquatic pollution is a challenge, and finding eco-friendly strategies could help to overcome such a problem. Herein, we studied the antagonistic potential of dietary fortification of white poplar (Populus alba; PA) leaf powder against chronic hexaflumuron (HX) toxicity in Nile tilapia (Oreochromis niloticus). Fish (n = 200; 36.20 ± 1.55 g) were eventually grouped into four groups with five replicates and kept for 60 days. The C (control) and PA groups were fed basal diets fortified with 0 and 6 g PA/kg diet, respectively, without toxicant exposure. Additionally, the HX and PA + HX groups were exposed to 1/10 of 96-h lethal concentration 50 (96-h LC<sub>50</sub>) of HX (0.72 mg/L) and given the same diets as those of the C and PA groups, respectively. The biochemical, immune-antioxidant, survival, splenic gene expression, and tissue microstructure were assessed at the end of the exposure time. The outcomes of this research showed that exposure to HX resulted in biochemical disorders (elevated blood glucose, cortisol, alanine aminotransferase, aspartate aminotransferase, and creatinine) in Nile tilapia. Immune suppression (lowered complement 3 and immunoglobulin M) and oxidative stress (lowered superoxide dismutase and catalase activity and higher malondialdehyde) were consequences of HX toxicity. The splenic expression of nuclear factor-kappa β65, kelch-like ECH-associated protein 1, and heme oxygenase-1 was down-regulated by HX exposure. Various pathological changes were noted as consequences of HX exposure in the liver, kidney, and spleen tissues. By feeding on the PA diet, the fish survivability was increased (90%) compared to the non-fed group (76%). Additionally, the biochemical disorders were modulated, and immune responses were enhanced due to PA feeding. Amelioration of the oxidative stress condition (by improving the antioxidant enzyme activity and lowering malondialdehyde) and the immune gene expression were noticed when the HX-exposed Nile tilapia were fed on the PA diet. A noticeable soothing effect was noticed by feeding on the PA diet against the pathological changes in the Nile tilapia tissues. Overall, feeding on a 6 g PA/kg diet ameliorates the detrimental consequences of HX toxicity in Nile tilapia.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 2","pages":"67"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish Physiology and Biochemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10695-025-01465-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Contamination of the aquatic bodies with pesticides is a serious issue that hinders the aquaculture industry worldwide. Preventing aquatic pollution is a challenge, and finding eco-friendly strategies could help to overcome such a problem. Herein, we studied the antagonistic potential of dietary fortification of white poplar (Populus alba; PA) leaf powder against chronic hexaflumuron (HX) toxicity in Nile tilapia (Oreochromis niloticus). Fish (n = 200; 36.20 ± 1.55 g) were eventually grouped into four groups with five replicates and kept for 60 days. The C (control) and PA groups were fed basal diets fortified with 0 and 6 g PA/kg diet, respectively, without toxicant exposure. Additionally, the HX and PA + HX groups were exposed to 1/10 of 96-h lethal concentration 50 (96-h LC50) of HX (0.72 mg/L) and given the same diets as those of the C and PA groups, respectively. The biochemical, immune-antioxidant, survival, splenic gene expression, and tissue microstructure were assessed at the end of the exposure time. The outcomes of this research showed that exposure to HX resulted in biochemical disorders (elevated blood glucose, cortisol, alanine aminotransferase, aspartate aminotransferase, and creatinine) in Nile tilapia. Immune suppression (lowered complement 3 and immunoglobulin M) and oxidative stress (lowered superoxide dismutase and catalase activity and higher malondialdehyde) were consequences of HX toxicity. The splenic expression of nuclear factor-kappa β65, kelch-like ECH-associated protein 1, and heme oxygenase-1 was down-regulated by HX exposure. Various pathological changes were noted as consequences of HX exposure in the liver, kidney, and spleen tissues. By feeding on the PA diet, the fish survivability was increased (90%) compared to the non-fed group (76%). Additionally, the biochemical disorders were modulated, and immune responses were enhanced due to PA feeding. Amelioration of the oxidative stress condition (by improving the antioxidant enzyme activity and lowering malondialdehyde) and the immune gene expression were noticed when the HX-exposed Nile tilapia were fed on the PA diet. A noticeable soothing effect was noticed by feeding on the PA diet against the pathological changes in the Nile tilapia tissues. Overall, feeding on a 6 g PA/kg diet ameliorates the detrimental consequences of HX toxicity in Nile tilapia.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
求助全文
约1分钟内获得全文 去求助
来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
期刊最新文献
Evaluating the dietary effects of curry and/or marigold leaf extracts on physiological and immunological responses of Labeo rohita and its resistance against Saprolegnia parasitica infection. Ameliorative potential of Populus alba leaf powder against hexaflumuron exposure in Nile tilapia: immune-antioxidant, biochemical, histological, and transcriptomic analysis. Carbonate alkalinity induces stress responses and renal and metabolic disorders in Nile tilapia: mitigation by camel whey protein hydrolysate diet. Effects of pyrimidine on cellular and neuronal arrangement, oxidative stress and energy content in the brain of the freshwater catfish, Heteropneustes fossilis. Development of colloidal gold test strip based on the BsVg819 gene fragment of vitellogenin of Bostrichthys sinensis for the detection of vitellogenin in multiple fish species.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1