p-chlorophenylalanine treatment accelerates tomato fruit ripening through hormone synthesis and glycolytic pathway during storage

IF 6.8 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2025-07-01 Epub Date: 2025-03-17 DOI:10.1016/j.postharvbio.2025.113517

Keyu Xu , Ran Yan , Hang Zhang , Lilai Yin , Wenbo Lei , Jianhui Cheng , Chen Huan , Yan Liu , Xiaolin Zheng , Shuling Shen

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Abstract

The accumulation of lycopene is the important indicator of tomato ripening and regulated by multiple plant signaling molecules. In this study, the effects of 30 μM p-chlorophenylalanine (p-CPA) on the postharvest ripening associated with lycopene content of tomato fruit were investigated during storage. The results showed that p-CPA treatment accelerated tomato fruit ripening, as indicated by increases in a* value, respiration rate and ethylene production, and decreases in firmness. p-CPA inhibited endogenous melatonin synthesis by competitive binding with tryptamine 5-hydroxylase (T5H), reducing the content of serotonin (the melatonin precursor), and down-regulating the genes expression of SlT5H, SlSNAT and SlASMT, while promoted ethylene synthesis and signal transduction by regulating the related genes. Meanwhile, p-CPA treatment enhanced the glycolytic pathway due to increasing the activity of 6-phosphofructokinase (PFK) and the contents of 3-phosphoglycerolaldehyde and pyruvate compared to the control. Also, p-CPA treatment enhanced the expressions of genes involved in lycopene synthesis including SlPSY1, SlPDS, SlZDS and SlCRTISO, thereby promoting the accumulation of lycopene. Thus, p-CPA treatment promoted tomato ripening along with lycopene accumulation by regulating endogenous melatonin and ethylene contents and activating glycolytic pathway during postharvest.

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对氯苯丙氨酸处理在贮藏过程中通过激素合成和糖酵解途径促进番茄果实成熟

番茄红素的积累是番茄成熟的重要指标，受多种植物信号分子的调控。研究了30 μM对氯苯丙氨酸（p-CPA）对番茄果实采后成熟及番茄红素含量的影响。结果表明，p-CPA处理加速了番茄果实的成熟，表现为a*值、呼吸速率和乙烯产量增加，硬度降低。p-CPA通过与色胺5-羟化酶（T5H）竞争结合，降低血清素（褪黑素前体）含量，下调SlT5H、SlSNAT和SlASMT基因表达，抑制内源性褪黑素合成，同时通过调节相关基因促进乙烯合成和信号转导。与此同时，p-CPA处理增加了6-磷酸果糖激酶（PFK）的活性以及3-磷酸甘油醛和丙酮酸的含量，从而增强了糖酵解途径。p-CPA处理还增强了参与番茄红素合成的基因SlPSY1、SlPDS、SlZDS和SlCRTISO的表达，从而促进了番茄红素的积累。因此，p-CPA处理通过调控内源褪黑素和乙烯含量，激活采后糖酵解途径，促进番茄成熟和番茄红素积累。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.