The Use of Cholesterol Inclusion Complexes Based on Randomly Methylated Beta-Cyclodextrins to Improve Human Sperm Cryotolerance

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2025-03-17 DOI:10.1134/S000635092470129X

A. G. Mironova, S. A. Yakovenko, S. I. Afanasyeva, A. N. Tikhonov, E. Yu. Simonenko

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Abstract

The cryoprotective properties of cholesterol inclusion complexes based on randomly methylated β-cyclodextrin (RAMEB) derivatives in relation to human sperm was studied. The interaction of various concentrations of RAMEB and cholesterol inclusion complexes based on RAMEB with sperm membranes was assessed. It was shown that the pronounced cytotoxicity of RAMEB against sperm is due to the interaction of cyclodextrins with lipid components of the membrane, in particular, cholesterol extraction. RAMEB complexes with cholesterol have significantly less pronounced cytotoxicity. A relationship has been established between the concentration of cholesterol in sperm membranes and the concentration of RAMEB complexes with cholesterol in the sample. It found that incubation of sperm before cryopreservation with 2 mg/mL of RAMEB complexes with cholesterol significantly increased survival (by 13.8%) and the proportion of progressively motile sperm (by 14.8%) after thawing. X-ray diffraction analysis showed that the presence of RAMEB and RAMEB complexes with cholesterol in the samples at the studied concentrations (2, 4, and 8 mg/mL) did not affect the average size of ice crystals formed in the sample during cryopreservation. The mechanism of cryoprotective effect of RAMEB complexes with cholesterol was proposed.

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使用基于随机甲基化β -环糊精的胆固醇包合物提高人类精子的低温耐受性

研究了随机甲基化β-环糊精（RAMEB）衍生物胆固醇包合物对人类精子的冷冻保护性能。评估了不同浓度的RAMEB和基于RAMEB的胆固醇包合物与精子膜的相互作用。结果表明，RAMEB对精子具有明显的细胞毒性是由于环糊精与膜的脂质成分，特别是胆固醇提取物的相互作用。RAMEB复合物与胆固醇的细胞毒性显著降低。已经建立了精子膜中胆固醇浓度与样品中RAMEB复合物与胆固醇浓度之间的关系。研究发现，在冷冻保存前用2mg /mL含胆固醇的RAMEB复合物孵育精子，显著提高了存活率（13.8%）和解冻后精子逐渐活动的比例（14.8%）。x射线衍射分析表明，在研究浓度（2、4和8 mg/mL）下，样品中RAMEB和RAMEB与胆固醇复合物的存在不影响样品在冷冻保存过程中形成的冰晶的平均大小。探讨了RAMEB复合物与胆固醇的低温保护作用机制。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.