{"title":"Mitochondrial Genomes of Six Discogobio Species (Teleostei, Cyprinidae) and Their Phylogenetic Analysis","authors":"Huan Cheng, Renrong Huang, Hongmei Li, Zhenya Qiu, Shan Xiong, Renyi Zhang","doi":"10.1002/ece3.71142","DOIUrl":null,"url":null,"abstract":"<p><i>Discogobio</i> is an important small freshwater economic fish in Southwestern China. In this study, we determined the complete mitochondrial genome (mitogenome) sequences of six <i>Discogobio</i> species by conventional overlapping PCR and Sanger sequencing. The mitogenomes were 16,591–16,605 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a noncoding region, which was consistent with previously studied Labeoninae mitogenomes. The phylogenetic analysis revealed that the subfamily Labeoninae was divided into four major clades. Notably, <i>Discogobio</i> was observed to be a non-monophyletic group and mixed with <i>Discocheilus</i>. The divergence time of <i>Discogobio</i> and <i>Discocheilus</i> can be placed during the time of the most recent common ancestor in the late Miocene (7.80 Mya). The results of the selection pressure analyses indicated that all <i>Discogobio</i> fishes exhibited Ka/Ks ratios < 1, suggesting that mitochondrial function in this genus was subjected to strong purifying selection and adapted to different environments. These mitogenomes will facilitate further studies in phylogeny, taxonomy, and evolutionary biology related to the subfamily Labeoninae.</p>","PeriodicalId":11467,"journal":{"name":"Ecology and Evolution","volume":"15 3","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ece3.71142","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecology and Evolution","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ece3.71142","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Discogobio is an important small freshwater economic fish in Southwestern China. In this study, we determined the complete mitochondrial genome (mitogenome) sequences of six Discogobio species by conventional overlapping PCR and Sanger sequencing. The mitogenomes were 16,591–16,605 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a noncoding region, which was consistent with previously studied Labeoninae mitogenomes. The phylogenetic analysis revealed that the subfamily Labeoninae was divided into four major clades. Notably, Discogobio was observed to be a non-monophyletic group and mixed with Discocheilus. The divergence time of Discogobio and Discocheilus can be placed during the time of the most recent common ancestor in the late Miocene (7.80 Mya). The results of the selection pressure analyses indicated that all Discogobio fishes exhibited Ka/Ks ratios < 1, suggesting that mitochondrial function in this genus was subjected to strong purifying selection and adapted to different environments. These mitogenomes will facilitate further studies in phylogeny, taxonomy, and evolutionary biology related to the subfamily Labeoninae.
期刊介绍:
Ecology and Evolution is the peer reviewed journal for rapid dissemination of research in all areas of ecology, evolution and conservation science. The journal gives priority to quality research reports, theoretical or empirical, that develop our understanding of organisms and their diversity, interactions between them, and the natural environment.
Ecology and Evolution gives prompt and equal consideration to papers reporting theoretical, experimental, applied and descriptive work in terrestrial and aquatic environments. The journal will consider submissions across taxa in areas including but not limited to micro and macro ecological and evolutionary processes, characteristics of and interactions between individuals, populations, communities and the environment, physiological responses to environmental change, population genetics and phylogenetics, relatedness and kin selection, life histories, systematics and taxonomy, conservation genetics, extinction, speciation, adaption, behaviour, biodiversity, species abundance, macroecology, population and ecosystem dynamics, and conservation policy.