Chang-Hyeock Byeon, Ted Kinney, Dr. Hakan Saricayir, Kasper Holst Hansen, Dr. Faith J. Scott, Sadhana Srinivasa, Meghan K. Wells, Dr. Frederic Mentink-Vigier, Prof. Wook Kim, Prof. Ümit Akbey
{"title":"Frontispiz: Ultrasensitive Characterization of Native Bacterial Biofilms via Dynamic Nuclear Polarization-Enhanced Solid-State NMR","authors":"Chang-Hyeock Byeon, Ted Kinney, Dr. Hakan Saricayir, Kasper Holst Hansen, Dr. Faith J. Scott, Sadhana Srinivasa, Meghan K. Wells, Dr. Frederic Mentink-Vigier, Prof. Wook Kim, Prof. Ümit Akbey","doi":"10.1002/ange.202581261","DOIUrl":null,"url":null,"abstract":"<p>Ultrasensitive characterization of native bacterial biofilm: A high-sensitivity dynamic nuclear polarization (DNP) enhanced solid-state NMR method illuminates the composition of intact native bacterial biofilm. The DNP sensitivity boost (~75-fold) allows observing NMR signals of biofilms composed of bacteria and extracellular matrix at natural abundance, shown here for Pseudomonas. Chemical identification quantifies the biofilm components, as reported by Ümit Akbey et^^al. in their Research Article (e202418146).\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure>\n </p>","PeriodicalId":7803,"journal":{"name":"Angewandte Chemie","volume":"137 12","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ange.202581261","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Angewandte Chemie","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ange.202581261","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Ultrasensitive characterization of native bacterial biofilm: A high-sensitivity dynamic nuclear polarization (DNP) enhanced solid-state NMR method illuminates the composition of intact native bacterial biofilm. The DNP sensitivity boost (~75-fold) allows observing NMR signals of biofilms composed of bacteria and extracellular matrix at natural abundance, shown here for Pseudomonas. Chemical identification quantifies the biofilm components, as reported by Ümit Akbey et^^al. in their Research Article (e202418146).