Assessing Bioavailability and the Toxicity of Resveratrol Nanoparticles: Insights from an In Vivo Chicken Embryonic Model.

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY Food and Chemical Toxicology Pub Date : 2025-03-14 DOI:10.1016/j.fct.2025.115390

Jinglin Zhang, Xinwen Zhang, Qin Wang, Changqing Wu

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引用次数: 0

Abstract

This study evaluates the bioavailability, developmental toxicity, genotoxicity, and estrogenic effects (EA) of resveratrol nanoparticles (NPs) stabilized by Tween 80 and carboxymethyl chitosan (CMCS) using chicken embryonic models. Tween NPs exhibited smaller sizes (93.18 nm), and more homogeneous distribution compared to CMCS NPs (267.92 nm). Resveratrol-loaded CMCS NPs achieved a peak serum concentration (Cmax) of 0.462 μg/mL at 60 minutes, while Tween NPs showed lower bioavailability (Cmax = 0.16 μg/mL). CMCS NPs induced a higher mortality (45.0%) at 760 μg/kg compared to Tween NPs (25.0%) and resveratrol (0%). Without loaded with resveratrol, Tween Empty NPs and CMCS Empty NPs showed higher mortality (51.5% and 60.7%, respectively). As capping agents, Tween 80 showed minimal mortality but induced malformation of 15.8% at higher dose. CMCS increased mortality (30%) and malformation (10%) at 10 mg/kg. Empty NPs induced higher lipoperoxidation and DNA damage than resveratrol-loaded NPs. Tween Empty NPs significantly elevated DNA damage (p < 0.0001), while CMCS Empty NPs exhibited no genotoxic effects. Estrogen-responsive apolipoprotein II (ApoII) gene expression was significantly upregulated in the Tween Empty group (9.51 ± 3.27-fold), indicating potential EA of the nanocarrier. These findings highlight both the NPs and nanocarriers must be evaluated in safety of nano-formulations.

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.