Making CRISPR-Dx Technology Work in Environmental Monitoring

Abstract

Figure 1. Challenges and strategies in CRISPR-diagnostic practices for environmental monitoring. The primary limitations include the presence of inhibitors in complex environmental matrices that interfere with CRISPR-Dx, the inherent risk of nucleic acid aerosol contamination, and the qualitative nature of CRISPR-Dx, which falls short of meeting quantitative detection requirements. Potential solutions involve advancing in situ purification, one-pot detection methods, and developing semiquantitative or quantitative detection techniques. Dong-Feng Liu is a Professor at the State Key Laboratory of Advanced Environmental Technology, Department of Environmental Science and Engineering, University of Science and Technology of China (USTC). He obtained his Ph.D. in 2014 from USTC. Dr. Liu is a recipient of the National Science Fund for Excellent Young Scholars. He now serves as an Editorial Board Member for ISME Communications and a Youth Editorial Board Member for Frontiers of Environmental Science & Engineering. His research interests include water pollution control and environmental biotechnology. The authors thank the National Natural Science Foundation of China (52322002, 52192684, and 52400255), the Major Science and Technology Projects of Anhui Province (202203a07020015), the Science Fund for Distinguished Young Scholars of Anhui Province (2208085J36), the National Funded Postdoctoral Research Program of China (GZC20232543), and the Fundamental Research Funds for the Central Universities (WK2060000099) for supporting this work. This article references 5 other publications. This article has not yet been cited by other publications.