Positive regulation of BBX11 by NAC053 confers stomatal and apoplastic immunity against bacterial infection in Arabidopsis

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-03-20 DOI:10.1111/nph.70096

Sheng Luo, Charles Tetteh, Zhiqiang Song, Cheng Zhang, Pinyuan Jin, Xingqian Hao, Yingjun Liu, Shating Ge, Jiao Chen, Keke Ye, Kang Wang, Ting Zhang, Huajian Zhang

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Abstract

Stomatal immunity and apoplastic immunity are critical for preventing microbial phytopathogenesis. However, the specific regulatory mechanisms of these resistances remain unclear.
In this study, a BBX11 transcription factor (TF) was identified in Arabidopsis and was found to participate in stomatal and apoplast immunity. Phenotypic, biochemical, and genetic analyses revealed that NAC053 contributed to Arabidopsis resistance against Pseudomonas syringae pv tomato DC3000 (Pst DC3000) by positively regulating BBX11.
BBX11 TF that was expressed constitutively in guard cells acts as a positive regulator of plant defense against Pst DC3000 through the suppression of coronatine (COR)-induced stomatal reopening, mitigating the virulence of COR and alleviating COR-triggered systemic susceptibility in the apoplast. BBX11 was found to be involved in PTI responses induced by flg22, such as stomatal closure, reactive oxygen species accumulation, MAPK activation, and callose deposition, thereby enhancing disease resistance. Yeast one-hybrid screening identified NAC053 as a potential TF that interacted with the promoter of BBX11. NAC053 also positively regulated resistance to Pst DC3000.
These findings underscore the significance of transcriptional activation of BBX11 by NAC053 in stomatal and apoplastic immunity against Pst DC3000, enhancing understanding of plant regulatory mechanisms in response to bacterial pathogens.

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New Phytologist 生物-植物科学

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5.30%

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728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.

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