Application of Cerium Oxide Nanozymes (CeONZs) in Human Pluripotent Stem Cell-Derived Cardiomyocytes.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2025-03-20 DOI:10.1007/7651_2025_606

Chengwen Hang, Huixin Guo, Mohamed S Moawad, Christie M Sayes, Yi-Han Chen, Jian Yang

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Abstract

Cardiovascular diseases (CVDs) are a leading cause of death globally. Excessive production of reactive oxygen species (ROS) is detrimental to cardiomyocytes (CMs), triggering inflammation, inducing cell death, disrupting calcium homeostasis, and leading to arrhythmia. Thus, ROS is considered a common pathological factor in CVDs. Although the efficacy of antioxidants targeting ROS is currently limited, nanotechnology offers opportunities to develop antioxidants with improved selectivity and bioavailability, which can effectively prevent or treat oxidative stress-related CVDs. Cerium oxide nanozymes (CeONZs) can efficiently scavenge excessive ROS by mimicking the activity of endogenous antioxidant enzymes. However, their nanosafety and efficacy in human CMs remain unclear, posing a critical issue to be addressed before clinical applications. Due to the scarcity of primary human CMs, human pluripotent stem cells (hPSCs) and their derived cardiomyocytes (hPSC-CMs) provide a valuable source for modeling CVDs and their therapeutic interventions. This chapter presents a preparation method for CeONZs and outlines the assessment of their biosafety and antioxidant efficacy in hPSC-CMs.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.