A process-based dynamic model for succinic acid production by Actinobacillus succinogenes: regulatory role of ATP/ADP balance.

IF 4 2区生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1512982

Emiliano Salucci, Fabrizio Cartenì, Francesco Giannino, Elisabetta de Alteriis, Francesca Raganati, Stefano Mazzoleni

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Abstract

Introduction: Succinic acid is an important chemical compound for biotechnological productions, being used as a basic platform to produce many industrial products in major business applications. It can be produced as fermentation end-product of anaerobic metabolism of different bacterial species, among which Actinobacillus succinogenes is largely used. Modeling microbial metabolic processes in controlled bioreactor systems is recognized as a useful tool to optimize growth conditions aimed at maximizing yield.

Methods: A novel model is presented based on System Dynamics approach in which the maintenance of the ATP/ADP balance is introduced as a key regulatory process of A. succinogenes metabolism.

Results and discussion: Model simulations accurately reproduce microbial growth and succinic acid production in anaerobic batch cultures at different initial glucose concentrations. Results reveal that the main limitations to maximal succinic acid production are glucose uptake restrictions and energy homeostasis costs (ATP/ADP balance) of the microbial population. The process-based modeling approach effectively describes the main metabolic processes and their regulation, providing a useful tool to define working conditions and overcome the criticalities of the SA fermentation process.

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琥珀酸放线菌产琥珀酸的过程动力学模型：ATP/ADP平衡的调节作用。

琥珀酸是一种重要的生物技术生产化合物，在主要的商业应用中被用作生产许多工业产品的基础平台。它可以作为不同细菌厌氧代谢的发酵终产物产生，其中琥珀酸放线杆菌被大量使用。在可控生物反应器系统中模拟微生物代谢过程被认为是优化生长条件以实现产量最大化的有用工具。方法：提出了一种基于系统动力学方法的新模型，该模型将ATP/ADP平衡的维持作为琥珀酸草代谢的关键调控过程。结果和讨论：模型模拟准确再现微生物生长和琥珀酸生产厌氧批培养在不同的初始葡萄糖浓度。结果表明，最大琥珀酸产量的主要限制因素是微生物群体的葡萄糖摄取限制和能量稳态成本（ATP/ADP平衡）。基于过程的建模方法有效地描述了主要代谢过程及其调控，为定义工作条件和克服SA发酵过程的临界提供了有用的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.